DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS
DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS
批准号:
8170741
负责人:
RON ORLANDO
金额:
$2.61万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-06-01 至 2011-05-31
关键词:
AffinityCationsComplex MixturesComputer Retrieval of Information on Scientific Projects DatabaseFundingGrantIndividualInstitutionMass Spectrum AnalysisMethodologyMethodsMolecularPolysaccharidesProtonsResearchResearch PersonnelResourcesSourceStructureSurfaceUnited States National Institutes of Healthcomparativeimprovedionizationmolecular massresearch studytechnique development
中文摘要
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
定量比较多糖的表达需要高度灵敏的方法来区分和识别具有微妙不同结构的单个多糖。质谱学在满足这些要求的同时,为复杂混合物的分析提供了一种快速可靠的方法,因此被广泛应用于血糖研究。使用MS对生物分子进行定量的一个限制是,不同分子物种的电离效率可能会显著不同,这取决于分析物的分子质量、质子/阳离子亲和力、表面活性、与分析物的电离竞争或干扰分析物电离的化合物的存在等因素。我们参与设计新的方法来提高比较血糖实验的准确性。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
The quantitative comparison of glycan expression requires highly sensitive methods that can distinguish and identify individual glycans with subtly different structures. Mass spectrometry (MS) fulfills these requirements while providing a rapid and reliable method to analyze complex mixtures, and therefore has been widely used in glycomic studies. One limitation of using MS for the quantitation of biomolecules is that the ionization efficiencies of distinct molecular species can differ significantly, depending on such factors as the analyte's molecular mass, proton/cation affinity, surface activity, the presence of compounds which compete with or interfere with the ionization of the analyte, etc. We are involved in devising new methodologies to improve the accuracy of comparative glycomic experiments.
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会议论文
Isotopically Labeled Heparan Sulfate Glycosaminoglycan Disaccharides for use as Internal Standards
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批准号:10080563
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项目类别:
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资助金额:$25.21万
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财政年份:2020
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负责人:RON ORLANDO
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依托单位:
CREATING ANTIBODIES TO ENABLE THE STUDY OF MYCOBACTERIUM TUBERCULOSIS INFECTIONS IN GUINEA PIGS
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批准号:10027950
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项目类别:
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资助金额:$65.32万
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财政年份:2019
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负责人:RON ORLANDO
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依托单位:
Generation of antibodies to monitor Notch O-fucosylation in vivo
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批准号:9200115
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项目类别:
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资助金额:$24.23万
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财政年份:2016
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负责人:RON ORLANDO
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依托单位:
Development of Isotopically Labeled Glycoproteins for use as Internal Standards
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批准号:9908593
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项目类别:
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资助金额:$35.26万
-
财政年份:2015
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负责人:RON ORLANDO
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依托单位:
Development of Isotopically Labeled Glycoproteins for use as Internal Standards
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批准号:8834650
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项目类别:
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资助金额:$27.03万
-
财政年份:2015
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负责人:RON ORLANDO
-
依托单位:
Site-specific O-GlcNAc MAbs: New Tools for Glycoproteomics
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批准号:8395145
-
项目类别:
-
资助金额:$32.27万
-
财政年份:2012
-
负责人:RON ORLANDO
-
依托单位:
Site-specific O-GlcNAc MAbs: New Tools for Glycoproteomics
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批准号:8546432
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项目类别:
-
资助金额:$30.16万
-
财政年份:2012
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负责人:RON ORLANDO
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依托单位:
GLYCOPROTEIN GLYCOMICS: EXPRESSION IN MOUSE ES CELLS & PROGENY
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批准号:8363005
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项目类别:
-
资助金额:$6.02万
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财政年份:2011
-
负责人:RON ORLANDO
-
依托单位:
COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA
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批准号:8363013
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项目类别:
-
资助金额:$6.02万
-
财政年份:2011
-
负责人:RON ORLANDO
-
依托单位:
DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS
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批准号:8363021
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项目类别:
-
资助金额:$3.44万
-
财政年份:2011
-
负责人:RON ORLANDO
-
依托单位:
PORCINE REPRODUCTIVE & RESPIRATORY SYNDROME VIRUS-ASSOCIATED GLYCANS
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批准号:8363119
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项目类别:
-
资助金额:$0.34万
-
财政年份:2011
-
负责人:RON ORLANDO
-
依托单位:
N- & O-GLYCANS OF RECOMBINANT HUMAN OVIDUCTAL GLYCOPROTEIN EXPRESSED IN HEK293
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批准号:8363118
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项目类别:
-
资助金额:$0.34万
-
财政年份:2011
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负责人:RON ORLANDO
-
依托单位:
GLYCOPROTEIN GLYCOMICS: EXPRESSION IN MOUSE ES CELLS & PROGENY
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批准号:8170724
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项目类别:
-
资助金额:$4.57万
-
财政年份:2010
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负责人:RON ORLANDO
-
依托单位:
COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA
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批准号:8170732
-
项目类别:
-
资助金额:$4.57万
-
财政年份:2010
-
负责人:RON ORLANDO
-
依托单位:
IDENTIFICATION OF CELL SURFACE MARKERS FOR HUMAN EMBRYONIC STEM CELLS
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批准号:8170791
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项目类别:
-
资助金额:$2.61万
-
财政年份:2010
-
负责人:RON ORLANDO
-
依托单位:
GLYCOPROTEIN GLYCOMICS: EXPRESSION IN MOUSE ES CELLS & PROGENY
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批准号:7955983
-
项目类别:
-
资助金额:$4.44万
-
财政年份:2009
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负责人:RON ORLANDO
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依托单位:
COMPARATIVE PROTEOMICS OF EMBRYONIC MOUSE STEM CELL DEVELOPMENT
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批准号:7955998
-
项目类别:
-
资助金额:$4.44万
-
财政年份:2009
-
负责人:RON ORLANDO
-
依托单位:
COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA
-
批准号:7955999
-
项目类别:
-
资助金额:$4.44万
-
财政年份:2009
-
负责人:RON ORLANDO
-
依托单位:
DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS
-
批准号:7956013
-
项目类别:
-
资助金额:$2.53万
-
财政年份:2009
-
负责人:RON ORLANDO
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依托单位:
PROTEOMIC ANALYSIS OF CAST CUTICLES FROM ANOPHELES GAMBIAE
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批准号:7722652
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项目类别:
-
资助金额:$0.38万
-
财政年份:2008
-
负责人:RON ORLANDO
-
依托单位:
海外基金