DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 定量比较多糖的表达需要高度灵敏的方法来区分和识别具有微妙不同结构的单个多糖。质谱学在满足这些要求的同时，为复杂混合物的分析提供了一种快速可靠的方法，因此被广泛应用于血糖研究。使用MS对生物分子进行定量的一个限制是，不同分子物种的电离效率可能会显著不同，这取决于分析物的分子质量、质子/阳离子亲和力、表面活性、与分析物的电离竞争或干扰分析物电离的化合物的存在等因素。我们参与设计新的方法来提高比较血糖实验的准确性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The quantitative comparison of glycan expression requires highly sensitive methods that can distinguish and identify individual glycans with subtly different structures. Mass spectrometry (MS) fulfills these requirements while providing a rapid and reliable method to analyze complex mixtures, and therefore has been widely used in glycomic studies. One limitation of using MS for the quantitation of biomolecules is that the ionization efficiencies of distinct molecular species can differ significantly, depending on such factors as the analyte's molecular mass, proton/cation affinity, surface activity, the presence of compounds which compete with or interfere with the ionization of the analyte, etc. We are involved in devising new methodologies to improve the accuracy of comparative glycomic experiments.