DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 聚糖表达的定量比较需要高灵敏度的方法，这些方法可以区分和鉴定具有细微不同结构的单个聚糖。 质谱法（MS）满足了这些要求，同时提供了一种快速可靠的方法来分析复杂的混合物，因此已被广泛用于糖组学研究。 使用MS定量生物分子的一个限制是不同分子种类的电离效率可能显著不同，这取决于诸如分析物的分子质量、质子/阳离子亲和力、表面活性、与分析物的电离竞争或干扰分析物的电离的化合物的存在、我们参与设计新的方法，以提高比较糖组学实验的准确性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The quantitative comparison of glycan expression requires highly sensitive methods that can distinguish and identify individual glycans with subtly different structures. Mass spectrometry (MS) fulfills these requirements while providing a rapid and reliable method to analyze complex mixtures, and therefore has been widely used in glycomic studies. One limitation of using MS for the quantitation of biomolecules is that the ionization efficiencies of distinct molecular species can differ significantly, depending on such factors as the analyte's molecular mass, proton/cation affinity, surface activity, the presence of compounds which compete with or interfere with the ionization of the analyte, etc. We are involved in devising new methodologies to improve the accuracy of comparative glycomic experiments.