CRYOEM OF BACTERIAL TRANSCRIPTION ACTIVATION COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分解代谢激活蛋白(CAP；也称为cAMP受体蛋白，CRP)在大肠杆菌中激活了100多个启动子的转录。CAP是一个45 kDa的同源二聚体，与靶启动子内或附近的特定DNA位点结合，增强450 kDa大肠杆菌RNA聚合酶全酶(RNAP)结合和启动转录的能力。 简单的依赖CAP的启动子--即根据CAP的DNA位点的位置和相应的转录激活机制，只需要CAP来激活转录的启动子可以分为两类。为了完成我们对CAP如何完成转录激活的理解，需要完整的CAP-holoRNAP-DNA启动子复合体的结构模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The catabolite activator protein (CAP; also known as the cAMP receptor protein, CRP) activates transcription at more than one hundred promoters in Escherichia coli. CAP, a 45 kDa homodimer, binds to specific DNA sites in or near target promoters and enhances the ability of the 450 kDa E. coli RNA polymerase holoenzyme (RNAP) to bind and initiate transcription. Simple CAP-dependent promoters--i.e. promoters that require only CAP for transcription activation--can be grouped into two classes based on the position of the DNA site for CAP and the corresponding mechanism for transcription activation. To complete our understanding of how CAP accomplishes transcription activation, full structural models for CAP-holoRNAP-DNA promoter complexes are required.