CRYOEM OF BACTERIAL TRANSCRIPTION ACTIVATION COMPLEXES

细菌转录激活复合物的冷冻

• 批准号: 8169664
• 负责人: CATHERINE L LAWSON
• 金额: $ 1.29万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169664
• 关键词: Binding; Complex; Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; Cyclic AMP Receptor Protein; DNA; DNA-Directed RNA Polymerase; Escherichia coli; Funding; Genetic Transcription; Grant; Holoenzymes; Institution; Positioning Attribute; Research; Research Personnel; Resources; Site; Source; Structural Models; Transcriptional Activation; United States National Institutes of Health; base; promoter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The catabolite activator protein (CAP; also known as the cAMP receptor protein, CRP) activates transcription at more than one hundred promoters in Escherichia coli. CAP, a 45 kDa homodimer, binds to specific DNA sites in or near target promoters and enhances the ability of the 450 kDa E. coli RNA polymerase holoenzyme (RNAP) to bind and initiate transcription. Simple CAP-dependent promoters--i.e. promoters that require only CAP for transcription activation--can be grouped into two classes based on the position of the DNA site for CAP and the corresponding mechanism for transcription activation. To complete our understanding of how CAP accomplishes transcription activation, full structural models for CAP-holoRNAP-DNA promoter complexes are required.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 分解代谢激活蛋白（CAP；也称为 cAMP 受体蛋白，CRP）可激活大肠杆菌中一百多个启动子的转录。 CAP 是一种 45 kDa 同二聚体，可与靶启动子内或附近的特定 DNA 位点结合，并增强 450 kDa 大肠杆菌 RNA 聚合酶全酶 (RNAP) 结合和启动转录的能力。 简单的 CAP 依赖性启动子——即仅需要 CAP 进行转录激活的启动子可根据 CAP DNA 位点的位置和相应的转录激活机制分为两类。为了完成我们对 CAP 如何完成转录激活的理解，需要 CAP-holoRNAP-DNA 启动子复合物的完整结构模型。