TULANE COBRE: MOLECULAR, IMAGING AND ANALYTIC CORE

TULANE COBRE：分子、成像和分析核心

• 批准号: 7959836
• 负责人: Luis Gabriel Navar
• 金额: $ 22.35万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959836
• 关键词: Agar Gel Electrophoresis; Albumins; Area; Bathing; Biology; Cell Culture Techniques; Cells; Centers of Research Excellence; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Creatinine; DNA; Doctor of Philosophy; Electrophoretic Mobility Shift Assay; Enzyme-Linked Immunosorbent Assay; Equipment; Fluorescence; Funding; Gene Targeting; Genomics; Genotype; Glass; Grant; Histology; Human; Image; Image Analysis; Immunohistochemistry; Incubators; Institution; Laboratories; Laboratory Research; Medical Research; Microscope; Mus; New Brunswick; Polyacrylamide Gel Electrophoresis; Power Sources; Promoter Regions; Proteins; RNA; Rattus; Reader; Renal Hypertension; Reporting; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Robot; Robotics; Sampling; Slide; Source; Specialist; Staining method; Stains; System; Systems Analysis; Tail; Time; Tissues; Training; United States National Institutes of Health; Water; Western Blotting; Work; digital imaging; instrument; microwave electromagnetic radiation; molecular imaging; professor; ultraviolet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular, Imaging, and Analytical Core Laboratory consist of 3 rooms and 9 components as follows: 1. Main Laboratory Workroom 1-1. DNA Workspace This space is dedicated for DNA work such as genotyping of gene-targeted mice and analyzing for promoter regions of target genes. A well-trained laboratory research technician will handle samples. He/She will extract genomic DNA from the snipped tails of gene-targeted mice and perform PCR. He/She will also set up agarose gel electrophoresis and report the final results to the corresponding investigators. Included major equipments are: Applied Biosystems GeneAmp PCR System, Fisher Scientific's dual chamber water bath, Sanyo's microwave, Fisher Scientific's power supply, Bio Rad's horizontal agarose gel-electrophoresis system, UVP's ultraviolet transilluminator, Fisher Scientific's portable centrifuge machine, and New Brunswick Scientific's incubator shaker. Postdoctoral Research Fellow: TBD #A (Ryo SATO, PhD) Laboratory Research Technician: TBD #B (G. Michael UPCHURCH, MS) 1-2. Western Blot/Gel Shift Assay Area This space is dedicated for western blot analysis/gel shift assay. A well-trained laboratory research technician will handle samples. He/She will extract protein samples from human/rats/mouse samples or cells provided by the investigators. He/She will also set up polyacrylamide gel electrophoresis and report the final results to the corresponding investigators. Included major equipments are: Licor's Odyssey system, Pharmacia Biotech's power supply and Novex's vertical PAGE system. Postdoctoral Research Fellow: Kayoko MIYATA, PhD Laboratory Research Technician: Nina A. PERRAULT, BS 1-3. Shared Open Space This open space is shared as a pipetting station. Included major equipments are: Denver Instrument's high quality scale, Thermolyne's platform shaker and Fisher Scientific's stirring hotplate. 1-4. Histology/Immunohistochemistry Workspace This space is dedicated for histology/immunohistochemistry works. A well-trained laboratory research technician will handle samples. He/She will set up slide glasses of human/rats/mouse samples provided by the investigators. He/She will also perform histology and/or immunohistochemistry staining and return the stained slides to the corresponding investigators. Included major equipments are: Dako Cytomation's Autostainer for immunohistochemistry robot, Fisher Scientific's chemical hume hood for straining, Fisher Scientific's slide oven. Postdoctoral Research Fellow: Naro OHASHI, MD, PhD Laboratory Research Technician: TBD #C 1-5. ELISA Station This space is dedicated for ELISA work. A well-trained laboratory research technician will handle samples. He/She will prepare protein samples for ELISA from human/rats/mouse samples provided by the investigators. He/She will also set up ELISA plates and report the final results to the corresponding investigators. Included major equipments are: Amersham Biosciences' ELISA plate washer, Boekel Scientific's ELISA plate shaker/incubator, BMG Labtech's FLUOstar Optima multi-detection microplate reader, and Bayer's DCA2000 creatinine/albumin auto-analyzer. Postdoctoral Research Fellow: TBD #D Laboratory Research Technician: Jessica L. MUCCI, MS 1-6. RNA Workspace This space is dedicated for RNA work such like RNA extraction and real-time RT-PCR. A well-trained laboratory research technician will handle samples. He/She will extract total RNA samples from human/rats/mouse samples or cells provided by the investigators using Qiagen's robotic system. He/She will also set up real-time RT-PCR and report the final results to the corresponding investigators. Included major equipments are: Qiagen's BioRobot EZ1, Amersham Biosciences' GeneQuant spectrometer, Fisher Scientific's portable centrifuge machine and Fisher Scientific's tissue homogenizer. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Michael UPCHURCH, MS 1-7. Analytical Equipment Station This space is dedicated for analytical equipments. Included major equipments are: Stratagene's real-time PCR machines (MX3000P and MX3005P) and Alphainotech's densitometric machine. 2. Cell Culture Workroom A separated room is dedicated for cell cultures. Included major equipments are: Labconco's purifier class II biosafety cabinet Heraeus's cell incubator, Fisher Scientific's inverted microscope and Eppendorf's bench-top refrigerated centrifuge machine. Postdoctoral Research Fellow: TBD #A (Ryo SATO, PhD) Laboratory Research Technician: TBD #B (G. Michael UPCHURCH, MS) 3. Microscope Workroom A separated room is dedicated for microscopes and image analyses. Included major equipments are: Olympus's (general) microscope equipped to real-time digital image capturing system, Olympus's fluorescence-ready microscope equipped to real-time digital image capturing system, and Image-Pro plus image handling/analyzing system. Assistant Professor: Maki URUSHIHARA, MD, PhD Laboratory Research Technician: TBD #C (Medical Research Specialist: Akemi SATO, MS)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子、成像和分析核心实验室由3个房间和9个组成部分组成，如下所示： 1.实验所主工作间 1-1.DNA工作区 这个空间专门用于DNA工作，如基因靶向小鼠的基因分型和分析目标基因的启动子区域。一名训练有素的实验室研究技术员将处理样本。他/她将从剪下的基因靶向小鼠的尾巴中提取基因组DNA，并进行PCR。他/她还将建立琼脂糖凝胶电泳法，并将最终结果报告给相应的调查人员。主要设备有：应用生物系统公司的GeneAmp聚合酶链式反应系统、Fisher Science的双室水浴、三洋的微波炉、Fisher Science的电源、Bio Rad的水平琼脂糖凝胶电泳仪、UVP的紫外线透光仪、Fisher Science的便携式离心机和New Brunswick Science的孵化器摇床。 博士后研究员：待定#A(佐藤亮，博士) 实验室研究技术员：待定#B(G.迈克尔·厄普丘奇，MS) 1比2。Western Blot/Gel Shift分析区域 这个空间专门用于蛋白质印迹分析/凝胶漂移分析。一名训练有素的实验室研究技术员将处理样本。他/她将从研究人员提供的人/鼠/鼠样本或细胞中提取蛋白质样本。他/她还将建立聚丙烯酰胺凝胶电泳法，并将最终结果报告给相应的调查人员。包括的主要设备有：LICOR的奥德赛系统、Pharmacia Biotech的电源和Novex的垂直寻呼系统。 博士后研究员：宫田佳子，博士 实验室研究技术员：尼娜·A·佩罗特，理学士 1比3。共享开放空间 这片空地被共享为吸液站。包括的主要设备有：丹佛仪器的优质秤、Thermolyne的平台振荡器和Fisher Science的搅拌热板。 1比4。组织学/免疫组织化学工作区 这个空间专门用于组织学/免疫组织化学工作。一名训练有素的实验室研究技术员将处理样本。他/她将设置由调查人员提供的人/鼠/鼠样本的载玻镜。他/她还将进行组织学和/或免疫组织化学染色，并将染色的玻片退回给相应的调查人员。主要设备包括：达科细胞自动染色免疫组织化学机器人，费希尔科学公司的化学休姆罩用于过滤，费希尔科学公司的幻灯片烤箱。 博士后研究员：Naro Ohashi，医学博士 实验室研究技术员：待定#C 1比5。ELISA站 这个空间专门用来做酶联免疫吸附试验。一名训练有素的实验室研究技术员将处理样本。他/她将从调查人员提供的人/鼠/鼠样本中准备用于ELISA的蛋白质样本。他/她还将建立ELISA卡，并将最终结果报告给相应的调查人员。主要设备包括：amersham Biosciences的ELISA卡洗板机，Boekel Science的ELISA板摇床/培养箱，BMG LabTech的FLUOstar Optima多检测微量平板读取器，以及拜耳的DCA2000型肌酐/白蛋白自动分析仪。 博士后研究员：待定#d 实验室研究技术员：杰西卡·L·穆奇 1比6。RNA工作区 这个空间专门用于RNA提取和实时RT-PCR等RNA工作。一名训练有素的实验室研究技术员将处理样本。他/她将使用乔根的机器人系统从研究人员提供的人类/大鼠/小鼠样本或细胞中提取总RNA样本。他/她还将建立实时RT-PCR，并将最终结果报告给相应的调查人员。主要设备包括：QIAGEN的BioRobot EZ1、amersham Bioscience的GeneQuant光谱仪、Fisher Science的便携式离心机和Fisher Science的组织匀浆机。 博士后研究员：佐藤亮，博士 实验室研究技术员：密歇根州G.迈克尔·厄普丘奇 1比7。分析设备站 这个空间是专门用来放置分析设备的。包括的主要设备有：Stratagene的实时PCR机(MX3000P和MX3005P)和Alphainotech的密度仪。 2.细胞培养工作室 一个单独的房间专门用于细胞培养。主要设备包括：Labinco的II类生物安全净化器、Heraeus的细胞孵化器、Fisher Science的倒置显微镜和埃彭多夫的台式冷冻离心机。 博士后研究员：待定#A(佐藤亮，博士) 实验室研究技术员：待定#B(G.迈克尔·厄普丘奇，MS) 3.显微镜工作室 一个单独的房间专门用来放显微镜和图像分析。主要设备包括：配备了实时数字图像采集系统的奥林巴斯(通用)显微镜，配备了实时数字图像采集系统的奥林巴斯荧光就绪显微镜，以及Image-Pro plus图像处理/分析系统。 助理教授：Maki Urushihara医学博士 实验室研究技术员：待定#C (医学研究专家：佐藤昭美，MS)