TULANE COBRE: MOLECULAR, IMAGING AND ANALYTIC CORE

TULANE COBRE：分子、成像和分析核心

• 批准号: 8360253
• 负责人: Luis Gabriel Navar
• 金额: $ 22.13万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360253
• 关键词: Agar Gel Electrophoresis; Area; Bathing; Biology; Cell Culture Techniques; Cells; Centers of Research Excellence; Chemicals; DNA; Doctor of Philosophy; Electrophoretic Mobility Shift Assay; Equipment; Fluorescence; Funding; Gene Targeting; Genomics; Genotype; Glass; Grant; Histology; Human; Image; Image Analysis; Immunohistochemistry; Incubators; Laboratories; Laboratory Research; Medical Research; Microscope; Mus; National Center for Research Resources; New Brunswick; Polyacrylamide Gel Electrophoresis; Power Sources; Principal Investigator; Promoter Regions; Proteins; RNA; Rattus; Renal Hypertension; Reporting; Research; Research Infrastructure; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Robot; Robotics; Sampling; Slide; Source; Specialist; Staining method; Stains; System; Systems Analysis; Tail; Time; Tissues; Training; United States National Institutes of Health; Water; Western Blotting; Work; cost; digital; digital imaging; microwave electromagnetic radiation; molecular imaging; ultraviolet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Molecular, Imaging, and Analytical Core Laboratory consists of 3 rooms and 9 parts as follows: 1. Main Laboratory Workroom 1-1. DNA Workspace This space is dedicated for DNA work such as genotyping of gene-targeted mice and analyzing for promoter regions of target genes. A well-trained laboratory research technician will handle samples. He/She will extract genomic DNA from the snipped tails of gene-targeted mice and perform PCR. He/She will also set up agarose gel electrophoresis and report the final results to the corresponding investigators. Included major equipment are: Applied Biosystems GeneAmp PCR System, Fisher Scientific's dual chamber water bath, Sanyo's microwave, Fisher Scientific's power supply, Bio Rad's horizontal agarose gel-electrophoresis system, UVP's ultraviolet transilluminator, Fisher Scientific's portable centrifuge machine, and New Brunswick Scientific's incubator shaker. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 1-2. Western Blot/Gel Shift Assay Area This space is dedicated for western blot analysis/gel shift assay. A well-trained laboratory research technician will handle samples. He/She will extract protein samplesfrom human/rats/mouse samples or cells provided by the investigators. He/She will also set up polyacrylamide gel electrophoresis and report the final results to the corresponding investigators. Included major equipments are: Licor's Odyssey system, Pharmacia Biotech's power supply and Novex's vertical PAGE system. Postdoctoral Research Fellow: Kayoko MIYATA, PhD Laboratory Research Technician: Salem I. ELKHAYAT, MS 1-3. Shared Open Space This open space is shared as a pipetting station. Included major equipment are: Next Advance Inc.'s Bullet Blender BLUE, Fisher Scientific's accuSpin Micro17R refrigerated microcentrifuge, Denver Instrument¿s high quality scale, Thermolyne's platform shaker and Fisher Scientific's stirring hotplate. 1-4. Histology/Immunohistochemistry Workspace This space is dedicated for histology/ immunohistochemistry works. A well-trained laboratory research technician will handle samples. He/She will set up slide glasses of human/rats/mouse samples provided by the investigators. He/She will also perform histology and/or immunohistochemistry staining and return the stained slides to the corresponding investigators. Included major equipment are: Dako Cytomation's Autostainer for immunohistochemistry robot, Fisher Scientific's chemical hume hood for straining, Fisher Scientific's slide oven. Postdoctoral Research Fellow: Masumi KAMIYAMA, PhD Laboratory Research Technician: Salem I. ELKHAYAT, MS 1-6. RNA Workspace This space is dedicated for RNA work such like RNA extraction and real-time RT-PCR.A well-trained laboratory research technician will handle samples. He/She will extract total RNA samples from human/rats/mouse samples or cells provided by the investigators using Qiagen's robotic system. He/She will also set up real-time RT-PCR and report the final results to the corresponding investigators. Included major equipments are: Thermo Scientific's NanoDrop 2000, Qiagen's BioRobot EZ1, Amersham Biosciences' GeneQuant spectrometer, Fisher Scientific's portable centrifuge machine and Fisher Scientific's tissue homogenizer. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 1-7. Analytical Equipment Station This space is dedicated for analytical equipments. Major equipment are: Stratagene's real-time PCR machines (MX3000P and MX3005P) and Alphainotech's densitometric machine. 2. Cell Culture Workroom A separated room is dedicated for cell cultures. Included major equipment are: Nexcelom Cellometer's Automated Cell Counter, Labconco's purifier class II biosafety cabinet Heraeus's cell incubator, Fisher Scientific's inverted microscope and Eppendorf's bench-top refrigerated centrifuge machine. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 3. Microscope Workroom A separate room is dedicated for microscopes and image analysis. Major equipment are: EVOS fl digital inverted microscope, Olympus's (general) microscope equipped to real-time digital image capturing system, Olympus's fluorescence-ready microscope equipped to real-time digital image capturing system, and Image-Pro plusimage handling/analyzing system. Postdoctoral Research Fellow: Masumi KAMIYAMA, PhD Medical Research Specialist: Akemi SATO, MS Laboratory Research Technician: Salem I. ELKHAYAT, MS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 分子、成像和分析核心实验室由以下3个房间和9个部分组成： 1.主实验室工作室 1-1. DNA工作区 这个空间专门用于DNA工作，如基因靶向小鼠的基因分型和靶基因的启动子区域分析。一名训练有素的实验室研究技术员将处理样品。他/她将从基因靶向小鼠剪下的尾巴中提取基因组DNA并进行PCR。他/她还将设置琼脂糖凝胶电泳，并将最终结果报告给相应的研究者。主要装备包括：Applied Biosystems GeneAmp PCR系统、Fisher Scientific的双室水浴、三洋的微波炉、Fisher Scientific的电源、Bio Rad的水平琼脂糖凝胶电泳系统、UVP的紫外透照仪、Fisher Scientific的便携式离心机和新玩法科学的培养箱振荡器。 博士后研究员：佐藤亮博士 实验室研究技术员：G。Mike UPCHURCH，MS 1-2.蛋白质印迹/凝胶迁移试验面积 该空间专用于蛋白质印迹分析/凝胶迁移试验。一名训练有素的实验室研究技术员将处理样品。他/她将从研究者提供的人/大鼠/小鼠样本或细胞中提取蛋白质样本。他/她还将设置聚丙烯酰胺凝胶电泳，并将最终结果报告给相应的研究者。主要设备包括：Licor的Odyssey系统、Pharmacia Biotech的电源和Novex的垂直PAGE系统。 博士后研究员：宫田嘉代子博士 实验室研究技术员：Salem I。Elkhayat，MS 1-3.共享开放空间 这个开放的空间被共享作为移液站。主要设备包括：Next Advance Inc.的Bullet Blender BLUE、Fisher Scientific的AccuSpin Micro 17 R冷冻微量离心机、Denver Instrument的高质量天平、Therapeutne的平台振荡器和Fisher Scientific的搅拌加热板。 1-4.组织学/免疫组织化学工作区 这个空间是专门为组织学/免疫组织化学工作。一名训练有素的实验室研究技术员将处理样品。他/她将设置研究者提供的人/大鼠/小鼠样本的载玻片。他/她还将进行组织学和/或免疫组织化学染色，并将染色的载玻片返还给相应的研究者。主要装备包括：Dako Cytomation的免疫组织化学自动染色机、Fisher Scientific的化学染色罩、Fisher Scientific的载玻片烘箱。 博士后研究员：Masumi Kamiyama，PhD 实验室研究技术员：Salem I。Elkhayat，MS 1-6. RNA工作区 这个空间专门用于RNA工作，如RNA提取和实时RT-PCR。训练有素的实验室研究技术人员将处理样品。他/她将使用Qiagen的机器人系统从研究者提供的人/大鼠/小鼠样本或细胞中提取总RNA样本。他/她还将建立实时RT-PCR，并将最终结果报告给相应的研究人员。主要设备包括：Thermo Scientific的NanoDrop 2000、Qiagen的BioRobot EZ 1、阿默舍姆Biosciences的GeneQuant光谱仪、Fisher Scientific的便携式离心机和Fisher Scientific的组织匀浆器。 博士后研究员：佐藤亮博士 实验室研究技术员：G。Mike UPCHURCH，MS 1-7.分析设备站 这个空间专门用于分析设备。主要设备有：Stratagene的实时PCR仪（MX3000 P和MX3005 P）和Alphainotech的光密度仪。 2.细胞培养工作室 一个单独的房间专门用于细胞培养。主要装备包括：Nexcelom Cellometer的自动细胞计数器、Labconco的II级生物安全柜、Heraeus的细胞培养箱、Fisher Scientific的倒置显微镜和Eppendorf的台式冷冻离心机。 博士后研究员：佐藤亮博士 实验室研究技术员：G。Mike UPCHURCH，MS 3.显微镜工作室 一个单独的房间专门用于显微镜和图像分析。主要设备有：EVOS fl数字倒置显微镜、配备有实时数字图像捕获系统的Olympus（通用）显微镜、配备有实时数字图像捕获系统的Olympus荧光就绪显微镜和Image-Pro plusimage处理/分析系统。 博士后研究员：Masumi Kamiyama，PhD 医学研究专家：Akemi SATO，MS 实验室研究技术员：Salem I。Elkhayat，MS