Isolation and Purification of Cancer Stem Cells Based on Functional Appraoch

基于功能方法的癌症干细胞的分离和纯化

• 批准号: 7965935
• 负责人: Itzhak Avital
• 金额: $ 29.47万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7965935
• 关键词: Cells; Cholangiocarcinoma; Goals; Malignant Neoplasms; Methodology; Organ; Pancreatic Adenocarcinoma; Primary carcinoma of the liver cells; Solid; base; cancer stem cell; cancer therapy; malignant breast neoplasm; malignant stomach neoplasm; melanoma; novel; self renewing cell; stem

In this project we investigate wide array of cancers: hepatocellular carcinoma, cholangiocarcinoma, pancreatic adenocarcinoma, breast cancer, melanoma, and gastric cancers This project has two prongs: One, where we use a functional approach to identify the "best" cancer stem cell usiong all previously described methodes and markers and subjecting them to a rigorous tesing methodology. Once identified we will try to target these cells via the specific marker in attempt to generate novel cancer therapy. Two, using a novel methodology we try to isolate the self renwing cells via asymmetric division in various cancers and determine whether these are the "fitest" cancer stem cells

在这个项目中，我们研究了各种各样的癌症：肝细胞癌、胆管癌、胰腺腺癌、乳腺癌、黑色素瘤和胃癌。这个项目有两个方面：一，我们使用功能方法来识别“最佳”的癌症干细胞，使用所有先前描述的方法和标记，并对它们进行严格的测试方法。一旦确定，我们将尝试通过特定标记靶向这些细胞，试图产生新的癌症治疗方法。第二，使用一种新的方法，我们试图通过不对称分裂在各种癌症中分离自我更新的细胞，并确定这些是否是“最适合”的癌症干细胞