Purification of a Novel Antibody Against Solid Organ Cancer Stem Cells

抗实体器官癌干细胞的新型抗体的纯化

• 批准号: 7733401
• 负责人: Itzhak Avital
• 金额: $ 23.74万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733401
• 关键词: Antibodies; Antigens; Cholangiocarcinoma; Detection; Goals; Malignant Neoplasms; Monoclonal Antibodies; Organ; Pancreatic Adenocarcinoma; Population; Predictive Value; Primary carcinoma of the liver cells; Proteins; Research; Screening for cancer; Solid; Testing; Therapeutic Agents; base; cancer stem cell; malignant stomach neoplasm; melanoma; novel; novel therapeutics; polyclonal antibody; tool

We are purifying populations of putative cancer stem cells from hepatocellular carcinoma, cholangiocarcinoma, pancreatic adenocarcinoma, melanoma and gastric cancer using a novel functional approach. We preliminarrily were able to generate a polyclonal antibody spesific for cancer stem cells. Now we are in the proccess of generating a monoclonal antibody. Subsequently, we will purify the specific protein and will test the antibody against a variety of various cancers. We hope to study the predictive value of this antibody as an early predictor.

我们正在使用一种新的功能方法纯化来自肝细胞癌、胆管癌、胰腺腺癌、黑色素瘤和胃癌的推定癌症干细胞群体。我们初步能够生成一种针对癌症干细胞的多克隆抗体。现在我们正在研制一种单克隆抗体。随后，我们将纯化特定的蛋白质，并将测试针对各种癌症的抗体。我们希望研究该抗体作为早期预测因子的预测价值。