Signal Processing And Instrumentation Section

信号处理和仪器部分（SPIS）的研究和开发活动是与NIH研究所科学家的合作努力，并经常导致独特的，专业的生物医学仪器的开发。其他项目涉及系统仿真和数据分析所需的信号和视频处理算法开发。SPIS的能力和成就使该小组成为NIH此类工程研究和技术开发的协调中心。示例技术和方法开发项目以及相关的研究包括： 1. 激光捕获组织显微切割（LCM）技术用于正常发育和病理的大分子分析 2. 表达组织显微切割（xMD）方法，其使得能够进行亚细胞分离以鉴定细胞器蛋白 3. 组织微阵列技术 4. 双光子激发荧光显微术（TPEFM）体内方法学 5. 荧光光激活定位显微镜（FPAL）纳米成像 6. 电子顺磁共振（EPR）方法学使得能够进行体内功能和生理成像 7. cDNA和蛋白质微阵列技术 8. 磁共振成像（MRI）和功能性MRI（fMRI）方法和装置 9. 用于分析假定的诊断和治疗放射性示踪剂的生物分布的γ照相机成像 10. 用于疾病检测、监测和引导手术的荧光成像 11. 用于组织定量表征的光学偏振成像和统计分析 12. 用于设施笼中定量行为评估的自动小鼠活动监测系统（MAMS） 13. 临床病理学组织固定和切片方法 14. 用于分子分析的微流控、微加工和微分析技术 15. 单分子、DNA和染色质纤维力学和操作技术 16. 时间和光谱可编程照明技术，用于健康和节奏的夹带 17. 使用个体化的基于MR的模型将体内前列腺MRI与组织病理学相关联 18. 果蝇视觉运动行为分析和色觉回路的遗传解剖方法 19. 行为神经生理学仪器和方法的自主测量 20. 用于细胞和组织的动力学、构象和组织特征的纳米级分析的振动光谱近场扫描显微镜成像 21. 用于癌症治疗的光动力疗法（PDT）技术 22. 语音采集、分析和实时自适应处理方法 23. 研究胎儿发育过程中心理、生理和行为过程的非人灵长类母胎监测技术 24. 生物力学实时测量与分析技术 25. 肌纤维张力瞬态测试与分析技术 26. 通过小鼠模型试验研究疼痛的遗传学和神经特异性传递机制的自动刺激和监测仪器 27. 用于分析细菌视紫红质能量转换机制的高速扫描光谱法 28. 实时多光谱内窥镜成像作为外科手术的辅助手段 29. 正电子发射断层成像 30. 共聚焦显微镜成像 31. 染色体显微切割技术 32. 用于评估内皮功能的非侵入性实时体内红外成像 33. 原子力显微镜成像 34. 磁共振弹性成像 35. 超声成像

The research and development activities of the Signal Processing and Instrumentation Section (SPIS) are collaborative efforts with NIH Institute scientists, and often result in the development of unique, specialized biomedical instruments. Other projects involve signal and video processing algorithm development required for system simulation and data analysis. SPIS capabilities and accomplishments have established the group as the focal point for this type of engineering research and technology development at the NIH. Example technology and methodology development projects, as well as associated research studies include: 1. laser capture tissue microdissection (LCM) technologies for macromolecular analysis of normal development and pathology 2. expression tissue microdissection (xMD) methodologies enabling subcellular isolation for identification of organelle proteins 3. tissue microarray (TMA) technologies 4. two-photon excitation fluorescence microscopy (TPEFM) in-vivo methodologies 5. fluorescence photo activation localization microscopy (FPALM) nanoscale imaging 6. electron paramagnetic resonance (EPR) methodologies enabling in vivo functional and physiological imaging 7. cDNA and protein microarray technologies 8. magnetic resonance imaging (MRI) and functional MRI (fMRI) methodologies and devices 9. gamma camera imaging for analyzing bio-distribution of putative diagnostic and therapeutic radiotracers 10. fluorescence imaging for disease detection, monitoring, and guided surgery 11. optical polarization imaging and statistical analysis for quantitative characterization of tissue 12. automated mouse activity monitoring system (MAMS) for quantitative behavioral assessment in facility cages 13. clinical pathology tissue fixation and sectioning methodologies 14. microfluidics, microfabrication, and microanalysis technologies for molecular analysis 15. single molecule, DNA, and chromatin fiber mechanics and manipulation technologies 16. temporal and spectral programmable lighting technologies for health and rhythm entrainment 17. correlating in vivo prostate MRI and histopathology using individualized MR-Based molds 18. fly optomotor behavioral analysis and genetic dissection of color-vision circuits methodologies 19. autonomic measures for behavioral neurophysiology instrumentation and methodologies 20. vibrational spectroscopic near-field scanning microscopy imaging for nanoscale analysis of dynamical, conformational and organizational characteristics of cells and tissues 21. photodynamic therapy (PDT) technologies for cancer treatment 22. speech acquisition, analysis, and real-time adaptive processing methodologies 23. nonhuman primate maternal-fetal monitoring technologies for investigation of psychological, physiological, and behavior processes during fetal development 24. biomechanics real-time measurement and analysis technologies 25. muscle fiber tension transient instrumentation and analysis technologies 26. automated stimulation and monitoring instrumentation for investigation of genetics and neuro-specific transmission mechanisms of pain via mouse model assays 27. high-speed scanning optical spectrometry for analysis of bacteriorhodopsin energy transduction mechanisms 28. real-time multispectral endoscope imaging as an aid to surgery 29. positron emission tomography (PET) imaging 30. confocal microscopy imaging 31. chromosome microdissection technologies 32. non-invasive real-time in-vivo infrared imaging for assessment of endothelial function 33. atomic force microscopy (AFM) imaging 34. magnetic resonance elastography (MRE) imaging 35. ultrasound imaging