A Walk-UP LC-MS for the Rapid Analysis of Organic Compounds

描述（由申请人提供）：一个液相色谱-质谱仪（LC-MS）是要求为这个共享仪器补助金申请。该仪器由一个高效液相色谱系统耦合到一个正交的飞行时间ESI/APCI质谱仪，并将被设置为直接的“开放式”仪器的研究人员（研究生，博士后研究员，本科生和研究技术人员）。对复杂分子样品进行化学表征的能力是各个学科的核心，特别是化学科学。该仪器的用户将主要由从事合成对生物学和人类医学具有重要意义的新型有机和有机金属分子的研究人员组成。一个多功能的LC-MS仪器配备了自动进样器和“步行”访问软件的学生操作将显着推进几个令人兴奋的NIH资助的研究项目。这些努力中的每一个都依赖于在每个合成过程中形成的分子产物的快速结构解析，因此直接依赖于研究人员立即可用的分子分析技术。开放式LC-MS仪器的可用性允许在进行反应时立即获得反应的质量分布数据，这不仅会增加反应的速度，而且还会提高科学评估每个反应的准确性。这将大大加快在制备具有生物学重要性的分子方面取得新发现的速度。NIH资助的相关项目包括但不限于开发用于制备具有生物学和治疗学意义的天然和非天然分子的新合成方法。这些目标涉及靶向和多样性导向的合成新的抗菌，抗病毒和抗肿瘤剂，各种天然和非天然产物，类固醇受体的分子探针，乳腺肿瘤成像的放射性药物，和生物医学传感器阵列的小分子蛋白质模拟物。 公共卫生相关性：伊利诺伊大学化学系的几位NIH资助的研究人员寻求资金购买一种用于液相色谱/质谱联用的仪器。这样的仪器将大大提高我们分析有机反应的能力，最终使我们的项目目标取得更快的进展。

DESCRIPTION (provided by applicant): . A Liquid Chromatography-Mass Spectrometer (LC-MS) is requested for this Shared Instrumentation Grant application. The instrument consists of a High Performance Liquid Chromatography system coupled to an orthogonal time-of-flight ESI/APCI mass spectrometer, and is to be set up as direct "open-access" instrumentation to researchers (graduate students, postdoctoral fellows, undergraduates, and research technicians). The ability to chemically characterize complex molecule samples lies at the heart of various disciplines, including the chemical sciences in particular. The users of this instrumentation will primarily consist of researchers engaged in the synthesis of novel organic and organometallic molecules that are of importance to biology and human medicine. A versatile LC-MS instrument equipped with an autosampler and "walk-on" access software for student operation will significantly advance several exciting NIH-funded research projects. Each of these efforts depends on the rapid structure elucidation of the molecular products formed in each synthetic process and is therefore directly dependent on the molecular analysis techniques immediately available to the researcher. The availability of open-access LC-MS instrumentation, which allows for immediate access to mass profile data of reactions as they are being performed, will not only increase the rate at which reactions can be performed, but also enhance the accuracy with which each reaction can be scientifically evaluated. This would greatly accelerate the rate at which novel discoveries can be made in the preparation of molecules of biological importance. The associated NIH-funded projects include, but are not limited to, the development of new synthetic methods for the preparation of natural and non-natural molecules of biological and therapeutical interest. These goals involve both target- and diversity-oriented synthesis of novel anti-bacterial, antiviral and antitumor agents, various natural and non-natural products, molecular probes for steroid receptors, radiopharmaceuticals for breast tumor imaging, and small molecule protein mimics for biomedical sensor arrays. PUBLIC HEALTH RELEVANCE: Several NIH-funded investigators in the Department of Chemistry at the University of Illinois seek funding to purchase an instrument for combined liquid chromatography/mass spectrometry. Such an instrument will significantly enhance our ability to analyze organic reactions, ultimately leading to much more rapid progress toward our project goals.