A Walk-UP LC-MS for the Rapid Analysis of Organic Compounds

用于快速分析有机化合物的直接 LC-MS

• 批准号: 7792972
• 负责人: Paul Hergenrother
• 金额: $ 29.93万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-15 至 2011-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792972
• 关键词: Anti-Bacterial Agents; Antiviral Agents; Applications Grants; Biological; Biological Factors; Chemicals; Chemistry; Complex; Computer software; Coupled; Data; Development; Discipline; Funding; Goals; Heart; High Pressure Liquid Chromatography; Human Biology; Illinois; Image; Liquid Chromatography; Mammary Neoplasms; Medicine; Methods; Molecular; Molecular Analysis; Molecular Probes; Postdoctoral Fellow; Preparation; Process; Proteins; Radiopharmaceuticals; Reaction; Research; Research Personnel; Research Project Grants; Sampling; Science; Steroid Receptors; Structure; Students; System; Techniques; Time; Universities; Walking; antitumor agent; graduate student; instrument; instrumentation; interest; liquid chromatography mass spectrometry; mass spectrometer; novel; operation; public health relevance; sensor; small molecule

DESCRIPTION (provided by applicant): . A Liquid Chromatography-Mass Spectrometer (LC-MS) is requested for this Shared Instrumentation Grant application. The instrument consists of a High Performance Liquid Chromatography system coupled to an orthogonal time-of-flight ESI/APCI mass spectrometer, and is to be set up as direct "open-access" instrumentation to researchers (graduate students, postdoctoral fellows, undergraduates, and research technicians). The ability to chemically characterize complex molecule samples lies at the heart of various disciplines, including the chemical sciences in particular. The users of this instrumentation will primarily consist of researchers engaged in the synthesis of novel organic and organometallic molecules that are of importance to biology and human medicine. A versatile LC-MS instrument equipped with an autosampler and "walk-on" access software for student operation will significantly advance several exciting NIH-funded research projects. Each of these efforts depends on the rapid structure elucidation of the molecular products formed in each synthetic process and is therefore directly dependent on the molecular analysis techniques immediately available to the researcher. The availability of open-access LC-MS instrumentation, which allows for immediate access to mass profile data of reactions as they are being performed, will not only increase the rate at which reactions can be performed, but also enhance the accuracy with which each reaction can be scientifically evaluated. This would greatly accelerate the rate at which novel discoveries can be made in the preparation of molecules of biological importance. The associated NIH-funded projects include, but are not limited to, the development of new synthetic methods for the preparation of natural and non-natural molecules of biological and therapeutical interest. These goals involve both target- and diversity-oriented synthesis of novel anti-bacterial, antiviral and antitumor agents, various natural and non-natural products, molecular probes for steroid receptors, radiopharmaceuticals for breast tumor imaging, and small molecule protein mimics for biomedical sensor arrays. PUBLIC HEALTH RELEVANCE: Several NIH-funded investigators in the Department of Chemistry at the University of Illinois seek funding to purchase an instrument for combined liquid chromatography/mass spectrometry. Such an instrument will significantly enhance our ability to analyze organic reactions, ultimately leading to much more rapid progress toward our project goals.

描述(由申请人提供)：此共享仪器拨款申请需要一台液质联用(LC-MS)。该仪器由一个与飞行时间正交型ESI/APCI质谱计连接的高效液相色谱系统组成，并将作为研究人员(研究生、博士后、本科生和研究技术人员)的直接“开放式”仪器而设置。对复杂分子样品进行化学表征的能力是各种学科的核心，特别是化学科学。这种仪器的使用者将主要由从事合成对生物学和人类医学具有重要意义的新型有机和有机金属分子的研究人员组成。一种多功能的LC-MS仪器配备了自动取样器和用于学生操作的“随身”访问软件，这将大大促进NIH资助的几个令人兴奋的研究项目。这些努力中的每一项都依赖于在每个合成过程中形成的分子产物的快速结构阐明，因此直接依赖于研究人员立即可用的分子分析技术。开放式LC-MS仪器的可用性允许在进行反应时立即访问大量的反应概况数据，这不仅将提高进行反应的速度，而且还可以提高对每个反应进行科学评估的准确性。这将大大加快在制备具有生物学意义的分子方面取得新发现的速度。NIH资助的相关项目包括但不限于，开发新的合成方法，以制备具有生物和治疗意义的天然和非天然分子。这些目标包括以靶向和多样性为导向合成新型抗菌、抗病毒和抗肿瘤药物、各种天然和非天然产品、类固醇受体分子探针、用于乳腺肿瘤成像的放射性药物以及用于生物医学传感器阵列的小分子蛋白质模拟物。 与公共健康相关：美国国立卫生研究院资助的伊利诺伊大学化学系的几名研究人员寻求资金，购买一种用于液相色谱/质谱分析相结合的仪器。这样的仪器将显著增强我们分析有机反应的能力，最终导致更快地实现我们的项目目标。