BD Canto II RUO Cytometer

BD Canto II RUO 细胞仪

• 批准号: 7793770
• 负责人: GARRY P NOLAN
• 金额: $ 23.55万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-17 至 2012-06-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793770
• 关键词: Acquired Immunodeficiency Syndrome; Clinical Research; Computer software; Custom; Development; Disease; Electronics; Failure; Fluorescence; Genetic; Lasers; Measures; Molecular Biology; Reader; Research; Risk; Signal Transduction; System; Training; United States National Institutes of Health; Work; base; cell analyzer; experience; flexibility; instrument; instrumentation; light scattering; operation; research study

DESCRIPTION (provided by applicant): We propose to purchase a three-laser Canto II research-only cell analyzer made by Becton, Dickinson and Company for the Stanford Shared FACS Facility. This system, which is capable of measuring two light scatter signals and up to eight fluorescence signals, will replace an analyzer custom built by Facility staff in 2000. The custom-built instrument now serves approximately 120 users from 35 different research labs at Stanford. The old analyzer is becoming increasingly difficult to maintain, in part due to difficulties in obtaining replacement parts, and poses an unacceptable risk of failure for ongoing experiments. Purchase of a new instrument will insure that Shared FACS Facility users have access to this critical instrumentation. The new purchase will not result in an increase in recharge rates to these users. The Canto II has a number of capabilities that are not available on the instrument to be replaced. These include two additional fluorescence parameters, 562-nm laser excitation, and a 96-well plate reader. These additions will allow current users enhanced flexibility in planning experiments and will provide current users of the Facility's high-end LSR-II analyzer the option of using a less-expensive instrument. The Shared FACS Facility staff has extensive experience with the software and electronics used in the Canto and should have no difficulties integrating it into the Facility and training the existing user base, as well as new users, in its operation. Almost all the work done in the Stanford Shared FACS Facility is health-related research, ranging from basic studies in molecular biology, genetics, and development to clinical research on AIDS and other diseases. The large majority of the work in the Facility is NIH supported.

描述(由申请人提供)：我们建议为斯坦福共享FACS设施购买由Becton，Dickinson and Company制造的三激光CANTO II仅供研究用的细胞分析仪。该系统能够测量两个光散射信号和多达八个荧光信号，将取代设备工作人员在2000年定制的分析仪。这种定制的仪器现在为斯坦福大学35个不同研究实验室的大约120名用户提供服务。旧的分析仪正变得越来越难维护，部分原因是难以获得更换部件，并对正在进行的实验造成不可接受的失败风险。购买一台新仪器将确保共享FACS设备用户能够访问这一关键仪器。新的购买不会导致对这些用户的充值费率增加。第二章有许多功能是要替换的乐器所不具备的。其中包括两个额外的荧光参数，562 nm激光激发和96孔板阅读器。这些增加将使目前的用户在规划实验方面具有更大的灵活性，并将为该设施的高端LSR-II分析仪的当前用户提供使用较便宜仪器的选择。共享FACS设施的工作人员对CATO中使用的软件和电子设备有丰富的经验，将其整合到设施中并培训现有用户基础和新用户操作应该没有困难。在斯坦福大学共享的FACS设施中完成的几乎所有工作都是与健康相关的研究，从分子生物学、遗传学和发育的基础研究到艾滋病和其他疾病的临床研究。该设施的大部分工作都得到了国家卫生研究院的支持。

项目成果

Dynamic changes in chromatin accessibility are associated with the atherogenic transitioning of vascular smooth muscle cells.

• DOI: 10.1093/cvr/cvab347
• 发表时间: 2021-11
• 期刊: Cardiovascular research
• 影响因子: 10.8
• 作者: Ying Wang;Hua Gao;Fudi Wang;Zhongde Ye;M. Mokry;A. Turner;Jianqin Ye;Simon Koplev;Lingfeng Luo;T. Alsaigh;S. Adkar;Maria Elishaev;Xiaotang Gao;L. Maegdefessel;J. Björkegren;G. Pasterkamp;Clint L. Miller;E. Ross;N. Leeper

Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cells.

II 类肽编辑器 H2-M 对 BCR 信号体的调节会影响先天样 B 细胞的发育和功能。

• DOI: 10.1016/j.celrep.2021.110200
• 发表时间: 2022
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Ghosh,Debopam;Pham,ThoD;Nanaware,PadmaP;Sengupta,Deepanwita;Adler,LitalN;Li,CaiyunG;He,Xiao;O'Mara,MaryE;Kantor,AaronB;Nguyen,KhoaD;Yang,Yang;Eisenlohr,LaurenceC;Jensen,PeterE;Herzenberg,LeonoreA;Stern,LawrenceJ;Bo
• 通讯作者: Bo

Gastric Mucosal Immune Profiling and Dysregulation in Idiopathic Gastroparesis.

• DOI: 10.14309/ctg.0000000000000349
• 发表时间: 2021-05-12
• 期刊: Clinical and translational gastroenterology
• 影响因子: 3.6
• 作者: Gottfried-Blackmore A;Namkoong H;Adler E;Martin B;Gubatan J;Fernandez-Becker N;Clarke JO;Idoyaga J;Nguyen L;Habtezion A
• 通讯作者: Habtezion A

Extracellular cGAMP is a cancer cell-produced immunotransmitter involved in radiation-induced anti-cancer immunity.

• DOI: 10.1038/s43018-020-0028-4
• 发表时间: 2020-02
• 期刊: Nature cancer
• 影响因子: 22.7
• 作者: Carozza JA;Böhnert V;Nguyen KC;Skariah G;Shaw KE;Brown JA;Rafat M;von Eyben R;Graves EE;Glenn JS;Smith M;Li L
• 通讯作者: Li L

Treatment with a neutrophil elastase inhibitor and ofloxacin reduces P. aeruginosa burden in a mouse model of chronic suppurative otitis media.

• DOI: 10.1038/s41522-021-00200-z
• 发表时间: 2021-04-06
• 期刊: NPJ biofilms and microbiomes
• 影响因子: 9.2
• 作者: Khomtchouk KM;Joseph LI;Khomtchouk BB;Kouhi A;Massa S;Xia A;Koliesnik I;Pletzer D;Bollyky PL;Santa Maria PL
• 通讯作者: Santa Maria PL