Thermo Fisher LTQ-Orbitrap XL Mass Spectrometer

Thermo Fisher LTQ-Orbitrap XL 质谱仪

• 批准号: 7791712
• 负责人: Michael MacCoss
• 金额: $ 50万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-13 至 2011-05-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791712
• 关键词: Address; Aging; Area; Biological Markers; Charge; Complex Mixtures; DNA Double Strand Break; Data; Exposure to; Faculty; Fourier Transform; Funding; Future; Grant; Hybrids; Ions; Isotopes; Life; Mass Spectrum Analysis; Measurement; Medicine; Molecular; Organism; Peptides; Post-Translational Protein Processing; Prevention; Process; Protein Analysis; Proteins; Proteomics; Research Project Grants; Resolution; Resources; Sampling; Speed; Technology; Toxic Environmental Substances; United States National Institutes of Health; Universities; Washington; college; genome sequencing; human disease; improved; instrument; instrumentation; mass spectrometer; novel; protein complex; public health relevance; reproductive

DESCRIPTION (provided by applicant): The enclosed proposal is requesting funds to expand the mass spectrometry and proteomics resources at the University of Washington through the purchase of a Thermo Fisher LTQ-Orbitrap mass spectrometer. This instrument will be invaluable in the successful completion of our existing NIH funded projects involving the qualitative and quantitative analysis of proteins and protein modifications of complex mixtures. Furthermore, a number of our projects are extending quantitative proteomics technology to the measurement of changes in the rate of turnover. Integral to the timely completion of these NIH research projects is the availability of a mass spectrometer capable of i) sub 5 ppm mass accuracy to distinguish between similar mass peptides, ii) a dynamic range of >1000 in a single spectrum, iii) data-dependent acquisition of tandem mass spectra with the sensitivity and speed of a quadrupole ion trap, and iv) the ability to resolve and detect unusual isotope distributions of multiply charged peptides within a complex mixture. Current mass spectrometers capable of these measurements are oversubscribed and, thus, can provide little to no access to our grants user groups. We propose to address these limitations through the acquisition of a shared hybrid mass spectrometer which combines the robustness and speed of a linear quadrupole ion-trap with the resolution, mass accuracy, and dynamic range of a Fourier transform mass spectrometer. The requested instrumentation will allow the faculty to address current technological limitations on a global level and improve the sample analysis bottleneck that will improve the capabilities of existing and future NIH funded projects within the University of Washington, College of Medicine. PUBLIC HEALTH RELEVANCE: We are requesting support to purchase a mass spectrometer for the qualitative and quantitative analysis of complex protein mixtures. These analyses will be used to improve our understanding of how a genome sequence encodes a functional living organism, to identify novel reproductive proteins, identify biomarkers of exposure to environmental toxins, understanding the molecular machinery responsible for double stranded DNA breaks, the basic molecular processes involved in aging, and more. All of these areas improve our understanding, prevention, or treatment of human disease.

描述（由申请人提供）：所附提案要求通过购买Thermo Fisher LTQ-Orbitrap质谱仪，提供资金以扩大华盛顿大学的质谱和蛋白质组学资源。该仪器将是非常宝贵的成功完成我们现有的NIH资助的项目，涉及定性和定量分析的蛋白质和蛋白质修饰的复杂混合物。此外，我们的一些项目正在将定量蛋白质组学技术扩展到测量周转率的变化。这些NIH研究项目的及时完成的组成部分是质谱仪的可用性，其能够i）低于5 ppm的质量精度以区分类似质量的肽，ii）在单个光谱中>1000的动态范围，iii）具有四极离子阱的灵敏度和速度的串联质谱的数据依赖性采集，和iv）分辨和检测复杂混合物中多电荷肽的异常同位素分布的能力。目前能够进行这些测量的质谱仪已被超额订购，因此几乎无法访问我们的赠款用户组。我们建议解决这些限制，通过收购一个共享的混合质谱仪相结合的鲁棒性和速度的线性四极离子阱的分辨率，质量精度和动态范围的傅里叶变换质谱仪。所要求的仪器将允许教师在全球范围内解决当前的技术限制，并改善样本分析瓶颈，这将提高华盛顿大学医学院现有和未来NIH资助项目的能力。 公共卫生相关性：我们正在请求支持购买一台质谱仪，用于复杂蛋白质混合物的定性和定量分析。这些分析将用于提高我们对基因组序列如何编码功能性活生物体的理解，识别新的生殖蛋白，识别暴露于环境毒素的生物标志物，了解负责双链DNA断裂的分子机制，衰老中涉及的基本分子过程等等。所有这些领域都提高了我们对人类疾病的理解、预防或治疗。