Purchase of a Triple Quadrupole Mass Spectrometry System for Metabolite Analysis

描述(由申请人提供)：该提案寻求资金用于购买安捷伦6460三重四极杆(QQQ)质谱仪系统，该系统将由美国国立卫生研究院(NIH)支持的威尔康奈尔医学院(WCMC)12名研究人员共享，用于生物分子的量化。该仪器满足了WCMC尚未满足的小分子定量的关键需求，并将通过包括在我们的MS核心设施中向研究人员广泛提供。值得注意的是，威尔康奈尔医学院的MS核心设施自1997年以来一直在运行，并且仍然是医学院及其附属医院(纽约长老会医院和特殊外科医院)MS仪器和支持的唯一来源。所要求的仪器将取代现有的三重四极杆MS，后者已有13年历史，无法用于我们的预期目的，原因包括灵敏度低、源设计排除了与LC系统的接口、无法进行高度多元化的多反应监测实验所需的快速转换以及无法利用离子源和操作/分析软件集成我们现有的高效液相色谱和质谱学系统进行代谢物分析。所要求的仪器将支持威尔·康奈尔对基础广泛的代谢物分析做出的坚定承诺，无论是有针对性的还是无针对性的，作为生物医学研究的变革性工具。正如研究主任随附的一封信中所记录的那样，WCMC在财政上承诺维护该仪器，并在其整个有用的生命周期内向NIH支持的生物医学科学家提供该仪器。 公共卫生相关性：此应用程序请求支持购买质谱仪，这将加强威尔·康奈尔医学研究所支持的研究人员的工作 大学来扩大我们对人类健康和疾病的了解。申请者通过使用所要求的仪器而瞄准的关键研究领域是癌症、传染病、干细胞生物学、糖尿病、动脉粥样硬化、结肠炎和神经生成。

DESCRIPTION (provided by applicant): This proposal seeks funds to purchase of an Agilent 6460 triple quadrupole (QqQ) mass spectrometry system that will be shared by 12 NIH-supported investigators at Weill Cornell Medical College (WCMC) for quantification of biomolecules. This instrument fulfills a critical unmet need at WCMC for small molecule quantification and will be made broadly available to investigators by inclusion in our MS core facility. Notably, the Weill Cornell Medical College MS core facility has been in operation since 1997 and continues to be the only source of MS instrumentation and support at the Medical College and its associated hospitals (New York Presbyterian Hospital and the Hospital for Special Surgery). The requested instrument will replace an existent triple-quadrupole MS that is now 13 years old and unusable for our intended purposes due to poor sensitivity, a source design that precludes interfacing with an LC system, inability to make the rapid transitions needed for highly-multiplexed multiple reaction monitoring experiments and inability to utilize ion sources and operating/analysis software that integrates our existing liquid chromatography and mass spectrometry systems for metabolite analyses. The requested instrument will support a solid commitment that Weill Cornell has made to broad-based Metabolite profiling, both targeted and untargeted, as a transformational tool for biomedical investigations. As documented in an accompanying letter from the Dean for Research, WCMC is financially committed to maintaining the instrument and making it available to NIH-supported biomedical scientists for its entire useful lifetime. PUBLIC HEALTH RELEVANCE: This application requests support for the purchase of a Mass Spectrometer that will enhance the efforts of NIH-supported researchers at Weill Cornell Medical College to expand our understanding of human health and disease. Key research areas that are being targeted by the applicants through use of the requested instrument are cancer, infectious diseases, stem cell biology, diabetes, atherosclerosis, colitis and neurogeneration.