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Doublecortin in Neuronal Migration

双皮质素在神经元迁移中的作用

基本信息

批准号：
8014907
负责人：
JOSEPH G GLEESON
金额：
$ 25.24万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-06-01 至 2015-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8014907
关键词：
Address Brain C-terminal Cells Centrosome Cerebral Palsy Clinical Complementary DNA Cortical Malformation Coupling Defect Development Electroporation Epilepsy Event Family Female Gene Family Genes Genetic Goals Homologous Gene Human Immigration In Vitro Knock-in Mouse Knock-out Lead Life Link Mediating Mediation Mental Retardation Methods Microtubule-Associated Proteins Microtubules Miller-Dieker Syndrome Molecular Movement Mus Mutate Mutation N-terminal Neurites Neurocognitive Deficit Neuronal Migration Disorder Neurons Nuclear Patients Phase Phenotype Phosphoric Monoester Hydrolases Phosphorylation Phosphotransferases Physical condensation Play Point Mutation Process Protein Dephosphorylation Protein-Serine-Threonine Kinases Proteins Regulation Role Seizures Signal Pathway Signal Transduction Syndrome Testing Travel Work Wrist adapter protein adult stem cell base cell motility cellular imaging human disease in utero in vivo infancy knock-down lissencephaly male migration mouse model neocortical neuron development neuronal cell body public health relevance relating to nervous system research study spinophilin

项目摘要

DESCRIPTION (provided by applicant): Malformations of cortical development due to disorder of neuronal migration are increasingly recognized as a common cause of epilepsy, mental retardation, and cerebral palsy. The doublecortin (DCX) gene is critical for neuronal migration in humans, as mutations result in X- linked lissencephaly in males and double cortex in females, producing severe neurocognitive deficits. We identified the DCX gene and found mutations in patients with this condition. We identified its role as a microtubule-associated protein (MAP) and its involvement in critical signaling pathways through phosphorylation- and dephosphorylation-dependent mechanisms. We uncovered important cellular roles for Dcx in cells, including mediation of nuclear-centrosome coupling, organization of microtubule condensation at the neurite "wrist" and a requirement in adult stem cell migration. Dcx is part of a gene family also containing Dclk1 and Dclk2, each encoding a strongly brain-expressed protein with a closely matching Dcx domain and kinase domain. We found that Dcx;Dclk1 knockouts displays severe cortical neuronal migration defects that mirror lissencephaly, whereas Dcx;Dclk2 knockouts displays severe seizures, also part of the clinical picture of lissencephaly. However, the role of the kinase activities in neuronal development are unknown. The overall goal of this renewal application is to elucidate the signaling mechanisms of the Dcx gene family in neuronal development and brain function. We will utilize knockout and knock-in and genetic rescue experiments in mice combined with advanced live-cell imaging capabilities and in vivo analysis that will synergize to provide a powerful approach to address these goals. PUBLIC HEALTH RELEVANCE: The doublecortin gene family plays critical roles in brain development, resulting in severe forms of epilepsy and mental retardation when mutated. We will study the signaling mechanisms of the doublecortin gene family, in order to understand the basis of these human diseases.

描述（由申请人提供）：由于神经元迁移障碍导致的皮质发育畸形越来越被认为是癫痫、智力低下和脑瘫的常见原因。双皮质素（DCX）基因对人类神经元迁移至关重要，因为突变导致男性X连锁无脑畸形和女性双皮质，产生严重的神经认知缺陷。我们确定了DCX基因，并在患有这种疾病的患者中发现了突变。我们确定了它作为微管相关蛋白（MAP）的作用，并通过磷酸化和去磷酸化依赖机制参与关键信号通路。我们发现了Dcx在细胞中的重要细胞作用，包括核-中心体偶联的介导，神经突“腕部”微管凝聚的组织以及成体干细胞迁移的要求。Dcx是Dclk 1和Dclk 2基因家族的一部分，Dclk 1和Dclk 2各自编码一种强脑表达蛋白，具有紧密匹配的Dcx结构域和激酶结构域。我们发现Dcx; Dclk 1基因敲除显示严重的皮质神经元迁移缺陷，反映无脑回畸形，而Dcx; Dclk 2基因敲除显示严重的癫痫发作，也是无脑回畸形临床表现的一部分。然而，激酶活性在神经元发育中的作用尚不清楚。这项更新申请的总体目标是阐明Dcx基因家族在神经元发育和脑功能中的信号传导机制。我们将利用小鼠中的敲除、敲入和遗传拯救实验，结合先进的活细胞成像能力和体内分析，这将协同作用，为实现这些目标提供一种强有力的方法。公共卫生相关性：doublecortin基因家族在大脑发育中起着关键作用，当突变时会导致严重形式的癫痫和智力迟钝。我们将研究doublecortin基因家族的信号传导机制，以了解这些人类疾病的基础。