Nuclear-cytoskeletal coupling in muscular laminopathies

肌肉核纤层蛋白病中的核细胞骨架耦合

• 批准号: 8044806
• 负责人: Jan Lammerding
• 金额: $ 2.11万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8044806
• 关键词: Adhesions; Affect; Biochemical; Biological Assay; Biomedical Engineering; Biopsy; Cell Nucleus; Cell physiology; Cells; Complex; Couples; Coupling; Cytoskeletal Modeling; Cytoskeleton; Data; Defect; Disease; Dominant-Negative Mutation; Duchenne muscular dystrophy; Dystrophin; Emery-Dreifuss Muscular Dystrophy; Etiology; Fibroblasts; Fracture; Gene Expression; Gene Silencing; Goals; Lamin Type A; Lamins; Lead; Limb-Girdle Muscular Dystrophies; Mammalian Cell; Measures; Mechanical Stimulation; Mechanics; Membrane Proteins; Methods; Modeling; Molecular; Molecular Biology Techniques; Mus; Muscle; Muscular Dystrophies; Mutation; Normal Cell; Normal tissue morphology; Nuclear; Nuclear Envelope; Patients; Phenotype; Positioning Attribute; Protein Family; Protein Isoforms; Proteins; RNA Interference; Research Personnel; Role; Signal Transduction; Striated Muscles; Techniques; Testing; Tissues; Work; base; design; env Gene Products; improved; insight; lamin C; magnetic beads; mouse model; mutant; novel; programs; response; tool; transmission process

DESCRIPTION (provided by applicant): Mutations in the nuclear envelope proteins lamins A and C cause Emery-Dreifuss muscular dystrophy, limbgirdle muscular dystrophy, and a broad spectrum of other diseases collectively called laminopathies. The underlying disease mechanism is unclear, but recent evidence suggests that disrupted nuclear-cytoskeletal coupling could contribute to the muscular phenotypes. Lamins A and C are required for the correct nuclear envelope localization of the LINC complex, which connects the nucleus to the cytoskeleton, but the effects of specific lamin A/C mutations remain unclear. My long term goal is to understand if lamin A/C mutations can disturb nuclear-cytoskeletal coupling through the LINC complex, resulting in abnormal intracellular force transmission and mechanotransduction that could contribute to the tissue specific phenotypes in muscular laminopathies, similar to the disease etiology of Duchenne muscular dystrophy. My specific aims are: 1. To test the hypothesis that the LINC complex is required for force transmission from the cytoskeleton to the nucleus. I will measure nuclear deformation in response to applied cytoskeletal strain after selective disruption of the LINC complex with dominant negative mutants or gene silencing by RNA interference. 2. To test the hypothesis that specific lamin A/C mutations can LINC complex localization to the nuclear envelope. I will analyze intracellular distribution and diffusional mobility of LINC complex components in cells from laminopathy patients, mouse models of muscular laminopathies, and healthy/wild-type controls. 3. To test the hypothesis that lamin A/C mutations can alter force transmission through the LINC complex, have designed a novel magnetic bead adhesion assay to quantify the maximal force that can be transmitted through the LINC complex and I will apply this technique to cells carrying specific lamin A/C mutations. 4. To test the hypothesis that disruption of the LINC complex alters mechanotransduction. I will measure strain-induced gene expression in cells in which LINC complex function has been selectively disrupted. Studying the effect of lamin A/C mutations on nuclear-cytoskeletal coupling will help to improve our understanding of normal and tissue-specific functions of the nuclear envelope and can lead to new insights into the molecular mechanisms responsible for muscular laminopathies such as Emery-Dreifuss muscular dystrophy, potentially providing new targets for the treatment of these diseases.

描述（由申请人提供）：核膜蛋白层蛋白A和C的突变导致emry - dreifuss肌营养不良症、肢带肌营养不良症和广泛的其他疾病，统称为层状病变。潜在的疾病机制尚不清楚，但最近的证据表明，核-细胞-骨骼偶联的破坏可能有助于肌肉表型。层粘连蛋白A和C是连接细胞核和细胞骨架的LINC复合体的正确核膜定位所必需的，但特异性层粘连蛋白A/C突变的影响尚不清楚。我的长期目标是了解层压蛋白A/C突变是否可以通过LINC复合物干扰核-细胞骨架偶联，从而导致细胞内力传递和机械转导异常，从而导致肌肉层压病的组织特异性表型，类似于杜氏肌营养不良的疾病病因。我的具体目标是：