Nuclear mechanics and mechanotransduction in muscular laminopathies

肌肉核纤层蛋白病的核力学和机械转导

• 批准号: 9067464
• 负责人: Jan Lammerding
• 金额: $ 40.25万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9067464
• 关键词: Actins; Address; Adrenergic Agonists; Adult; Affect; Animals; Biological Assay; Biopsy; Cardiac; Cardiac Myocytes; Cell Death; Cell Nucleus; Cell physiology; Cells; Clinical Treatment; Cytoplasm; Cytoplasmic Organelle; Cytoskeletal Proteins; Cytoskeleton; Data; Defect; Development; Dilated Cardiomyopathy; Disease; Drosophila genus; Drosophila melanogaster; Emery-Dreifuss Muscular Dystrophy; Event; Familial partial lipodystrophy; Fibroblasts; Gene Expression Regulation; Genes; Genetic; Goals; Health; Heart Abnormalities; Heart Contractilities; Heart Diseases; Heart Rate; Human; In Vitro; Inherited; Iowa; Lamin Type A; Lamins; Larva; Lead; Leg; Limb-Girdle Muscular Dystrophies; Link; Measures; Mechanical Stress; Mechanics; Modeling; Molecular; Muscle; Muscle Cells; Muscular Atrophy; Muscular Dystrophies; Mutate; Mutation; Myocardium; Myopathy; Normal tissue morphology; Nuclear; Nuclear Envelope; Nuclear Lamin; Nuclear Structure; Nuclear Translocation; Outcome; Pathway interactions; Patients; Phenotype; Play; Progressive Disease; Property; Proteins; Reporter; Reporting; Rupture; Serum Response Factor; Severities; Signal Pathway; Signal Transduction; Skeletal Muscle; Stress; Structure; Testing; Tissues; Universities; arm; design; disease phenotype; env Gene Products; factor A; fly; human disease; improved; in vivo; induced pluripotent stem cell; insight; lamin C; loris; mouse model; mutant; myocardin; novel; prevent; protein function; research study; response; transcription factor

DESCRIPTION (provided by applicant): More than one-third of all cases of dilated cardiomyopathy are caused by inherited mutations, with 5% to 10% of these mutations being linked to the LMNA gene, which encodes the nuclear envelope proteins lamin A and C. Importantly, mutations in the LMNA gene are also responsible for a broad spectrum of other diseases, including Emery-Dreifuss muscular dystrophy, limb-girdle muscular dystrophy and familial partial lipodystrophy. Despite recent advances, the mechanism(s) responsible for the often muscle-specific defects caused by different lamin mutations remains elusive. The central hypothesis of this proposal is that lamin mutations can cause skeletal and cardiac muscle disease through two, possibly overlapping mechanisms: (i) loss of structural function of lamins A and C, leading to rupture of the more fragile nucleus in mechanically stressed tissues; (ii) disturbing (mechanosensitive) signaling pathways that results in impaired function of muscle cells. Specific LMNA mutations may differentially affect these distinct aspects of lamin function, resulting in a broad spectrum of disease phenotypes. My long term goal is to understand the molecular mechanism(s) by which mutations in the nearly ubiquitously expressed lamins can lead to muscle-specific phenotypes and to explore to what extent impaired nuclear structure and altered cellular sensitivity to mechanical stress contribute to the muscle-specific phenotypes. In the first aim, we will test the hypothesis that altered nuclear mechanics result in increased nuclear rupture in mechanically stressed tissue. By using a genetic reporter assay that can detect even transiently compromised nuclear envelope integrity in cardiac myocytes in three mouse models of muscular laminopathies, we can directly assess whether mutations in nuclear envelope proteins cause increased rates of nuclear rupture in cardiac tissue. In the second aim, we will determine the relationship between impaired nuclear mechanics and the severity of muscular phenotypes in laminopathies. Using drosophila melanogaster models expressing a panel of lamin mutations with variable muscle involvement, we will relate effects of the mutations on the mechanical properties of nuclei in intact muscle tissue in drosophila larvae with the severity of muscle defects in adult flies. In the third aim, we will investigate the interplay between lamin mutations responsible for dilated cardiomyopathy and a specific signaling pathway, myocardin-related transcription factor A (MRTF-A). We will explore the mechanism(s) responsible for the impaired nuclear translocation of MRTF-A in lamin A/C-deficient and mutant cells we recently discovered and assess the functional consequences of impaired MRTF-A signaling on cellular function. Studying the effects of lamin mutations on nuclear structure and cellular signaling will improve our understanding of normal and tissue-specific functions of these proteins and lead to new insights into the molecular mechanisms responsible for dilated cardiomyopathy, Emery-Dreifuss muscular dystrophy and other laminopathies, potentially providing new targets for the treatment of these diseases.

描述（由申请人提供）：超过三分之一的扩张型心肌病病例是由遗传突变引起的，其中5%至10%的突变与LMNA基因有关，该基因编码核膜蛋白核纤层蛋白A和C。重要的是，LMNA基因的突变也是导致广泛的其他疾病的原因，包括Emery-Dreifuss肌营养不良症、肢带型肌营养不良症和家族性部分脂肪营养不良症。尽管最近取得了进展，但负责由不同核纤层蛋白突变引起的通常肌肉特异性缺陷的机制仍然难以捉摸。该提议的中心假设是，层粘连蛋白突变可以通过两种可能重叠的机制引起骨骼肌和心肌疾病：（i）层粘连蛋白A和C的结构功能丧失，导致机械应激组织中更脆弱的细胞核破裂;（ii）干扰（机械敏感）信号通路，导致肌肉细胞功能受损。特异性LMNA突变可能会差异性地影响核纤层蛋白功能的这些不同方面，导致广泛的疾病表型。我的长期目标是了解几乎无处不在表达的核纤层蛋白突变导致肌肉特异性表型的分子机制，并探索受损的核结构和改变的细胞对机械应力的敏感性在多大程度上有助于肌肉特异性表型。在第一个目标，我们将测试的假设，改变核力学的结果增加核破裂的机械应力组织。通过使用基因报告基因检测，即使是短暂受损的核膜完整性在三个小鼠模型的肌纤层蛋白病的心肌细胞，我们可以直接评估是否在核膜蛋白的突变导致心脏组织的核破裂率增加。在第二个目标中，我们将确定核力学受损与核纤层蛋白病肌肉表型严重程度之间的关系。使用果蝇模型表达一个面板的核纤层蛋白突变与可变的肌肉参与，我们将与果蝇幼虫的肌肉缺陷的严重程度，在完整的肌肉组织中的细胞核的机械性能的突变的影响。在第三个目标中，我们将调查核纤层蛋白突变负责扩张型心肌病和一个特定的信号通路，心肌相关转录因子A（MRTF-A）之间的相互作用。我们将探索我们最近发现的核纤层蛋白A/C缺陷和突变细胞中MRTF-A核转位受损的机制，并评估MRTF-A信号传导受损对细胞功能的功能后果。研究核纤层蛋白突变对核结构和细胞信号传导的影响将提高我们对这些蛋白质的正常和组织特异性功能的理解，并导致对扩张型心肌病，Emery-Dreifuss肌营养不良症和其他核纤层蛋白病的分子机制的新见解，可能为治疗这些疾病提供新的靶点。