Nuclear mechanics and mechanotransduction in muscular laminopathies
肌肉核纤层蛋白病的核力学和机械转导
基本信息
- 批准号:8413555
- 负责人:
- 金额:$ 7.94万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-01-01 至 2012-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): The nucleus is the distinguishing feature of eukaryotic cells and is separated from the surrounding cytoplasm by the nuclear envelope. Mutations in the nuclear envelope proteins lamin A/C and emerin cause Emery- Dreifuss muscular dystrophy, limb-girdle muscular dystrophy, familial partial lipodystrophy, and a variety of other diseases referred to as laminopathies. The underlying disease mechanism is unclear, in part because the function of the nuclear envelope proteins is incompletely defined. My long term goal is to understand the molecular mechanism(s) by which mutations in these ubiquitously expressed proteins can lead to such tissue-specific phenotypes. The central theme of this proposal is that muscle-specific phenotypes arise from specific abnormalities in nuclear envelope function, ranging from impaired structural function to abnormal transcriptional regulation, resulting in impaired adaptive and protective pathways. My specific aims are to: 1. Characterize the specific effects of mutations linked to Emery-Dreifuss muscular dystrophy on nuclear stability and cellular sensitivity to mechanical strain. Using recently established techniques, I will test the hypothesis that skin fibroblasts from Emery-Dreifuss muscular dystrophy patients have specifically impaired nuclear stability and abnormal regulation of mechanosensitive genes, resulting in decreased cell viability under strain. Cells from familial partial lipodystrophy patients and healthy control subjects will serve as controls for non-specific defects and normal nuclear envelope function, respectively. 2. Identify the muscle-specific effects of these mutations on nuclear mechanics and gene regulation. To test the hypothesis that tissue-specific defects in nuclear stability and mechanotransduction contribute to the muscular phenotype in Emery-Dreifuss muscular dystrophy, I will compare nuclear mechanics, strain- induced gene regulation, and cell viability under strain in muscle cells derived from mouse models of Emery- Dreifuss muscular dystrophy with fibroblasts from the same animals and with cells from wild-type littermates. Studying the specific cellular defects of these mutations will help to improve our understanding of normal and tissue-specific functions of the nuclear envelope and lead to new insights into the molecular mechanisms responsible for Emery-Dreifuss muscular dystrophy and other laminopathies such as limb-girdle muscular dystrophy, potentially providing new targets for the treatment of these diseases.
描述(由申请人提供):细胞核是真核细胞的显著特征,并通过核膜与周围的细胞质分离。核被膜蛋白核纤层蛋白A/C和Emerin中的突变引起Emery-Dreifuss型肌营养不良、肢带型肌营养不良、家族性部分脂肪营养不良和被称为核纤层蛋白病的多种其他疾病。潜在的疾病机制尚不清楚,部分原因是核膜蛋白的功能尚未完全确定。我的长期目标是了解这些普遍表达的蛋白质突变导致组织特异性表型的分子机制。该建议的中心主题是,肌肉特异性表型产生于核膜功能的特定异常,从受损的结构功能到异常的转录调控,导致适应性和保护性途径受损。我的具体目标是:1。表征与Emery-Dreifuss肌营养不良症相关的突变对核稳定性和细胞对机械应变敏感性的特定影响。使用最近建立的技术,我将测试的假设,从埃默里-德赖富斯肌营养不良症患者的皮肤成纤维细胞有专门受损的核稳定性和机械敏感基因的异常调节,导致应变下细胞活力下降。来自家族性部分脂肪营养不良患者和健康对照受试者的细胞将分别作为非特异性缺陷和正常核膜功能的对照。2.确定这些突变对核力学和基因调控的肌肉特异性影响。为了检验核稳定性和机械转导中的组织特异性缺陷有助于Emery-Dreifuss肌营养不良症中的肌肉表型的假设,我将比较来自Emery-Dreifuss肌营养不良症小鼠模型的肌细胞与来自相同动物的成纤维细胞和来自野生型同窝仔的细胞中的核力学、应变诱导的基因调控和应变下的细胞活力。研究这些突变的特定细胞缺陷将有助于提高我们对核膜正常和组织特异性功能的理解,并导致对Emery-Dreifuss肌营养不良症和其他核纤层蛋白病(如肢带型肌营养不良症)的分子机制的新见解,可能为治疗这些疾病提供新的靶点。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jan Lammerding其他文献
Jan Lammerding的其他文献
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{{ truncateString('Jan Lammerding', 18)}}的其他基金
2022 Intermediate Filaments Gordon Research Conference and Seminar
2022年中间长丝戈登研究会议暨研讨会
- 批准号:
10469043 - 财政年份:2022
- 资助金额:
$ 7.94万 - 项目类别:
Nuclear mechanobiology in confined migration (Equipment Supplement 2023)
受限迁移中的核力学生物学(设备增刊2023)
- 批准号:
10796133 - 财政年份:2020
- 资助金额:
$ 7.94万 - 项目类别:
Nuclear mechanics and mechanotransduction in muscular laminopathies
肌肉核纤层蛋白病的核力学和机械转导
- 批准号:
9067464 - 财政年份:2007
- 资助金额:
$ 7.94万 - 项目类别:
Nuclear mechanics and mechanotransduction in muscular laminopathies
肌肉核纤层蛋白病的核力学和机械转导
- 批准号:
7196846 - 财政年份:2007
- 资助金额:
$ 7.94万 - 项目类别:
Nuclear-cytoskeletal coupling in muscular laminopathies
肌肉核纤层蛋白病中的核细胞骨架耦合
- 批准号:
8044806 - 财政年份:2007
- 资助金额:
$ 7.94万 - 项目类别:
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