HIGHLY EFFECTIVE CONTROL OF AIDS VIRUS CHALLENGE IN MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 根据本方案，18只恒河猴被分配到三组之一。 在2009年4月、6月和8月用空载体（对照）、VSV/SFV E660或VSV/SFV E660 + GMCSF免疫动物。 所有动物均于2009年9月用SIVsmE 660攻毒。 接受对照疫苗的所有6只动物均被感染，血浆病毒载量峰值范围为6.0 - 8.2 log拷贝/ml。 3只对照动物的PVL在攻毒后稳步下降（其中2只目前低于可检测限），2只对照动物的PVL仍较高，在6.0 - 7.0 log拷贝/ml之间。 最后，一只对照动物因神经功能障碍被人道处死。 功能障碍的病因尚不清楚（组织学检查待定）。 接受VSV/SFV E660 + GMCSF的6只动物中有5只感染了峰值PVL，范围为4.7 - 7.8 log拷贝/ml。 其中一只动物目前的PVL低于可检测限度，该组中另一只动物的PVL在攻毒后稳步下降。 3只动物显示PVL保持稳定在4.0 - 6.0 log拷贝/ml之间。 接受VSV/SFV E660的6只动物中有2只感染了峰值PVL，分别为3.9和5.8 log拷贝/ml。 在攻毒后第35天和第42天，这两只动物的PVL均恢复至不可检测水平。 将继续监测所有动物，以跟踪感染或无感染的进展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Eighteen Rhesus macaques are assigned to one of three groups on this protocol. Animals were immunized with either empty vectors (controls), VSV/SFV E660 or VSV/SFV E660 + GMCSF in April, June and August 2009. All animals were challenged with SIVsmE660 in September 2009. All six animals receiving control vaccines became infected, with peak plasma viral loads ranging between 6.0 and 8.2 log copies/ml. PVL of three control animals has steadily declined after challenge (two of which are currently below detectable limits) and PVL of two control animals remains high between 6.0 and 7.0 log copies/ml. Finally one control animal was humanely euthanized due to neurologic dysfunction. The etiology of the dysfunction is not yet known (histology is pending). Five of the six animals receiving VSV/SFV E660 + GMCSF became infected with peak PVL ranging between 4.7 and 7.8 log copies/ml. One of these animals currently has PVL below detectable limits and the PVL of another animal in this group has steadily declined after challenge. Three animals exhibit PVL remaining steady between 4.0 and 6.0 log copies/ml. Two of six animals receiving VSV/SFV E660 became infected with peak PVL of 3.9 and 5.8 log copies/ml. The PVL of both of these animals returned to undetectable levels by day 35 and 42 post-challenge. All animals will continue to be monitored to follow the progression of infection or lack thereof.