DNA VACCINE FOR INDUCTION OF MUCOSAL IMMUNITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 实验1： 2008年，20只雌性印度猕猴被分配到四个试验组之一。所有动物之前都用DNA和各种趋化因子免疫了四次。根据从前四次免疫期间采集的样本获得的免疫学数据，决定进行第五次免疫。这种免疫将被修改为包括SIV nef/rev和H1N1 HA抗原。 在第五次免疫前三周(2009年10月26日至30日)，采集了所有20只动物的血液、粪便和BAL样本，并将其运往宾夕法尼亚大学。同时采集组织样本(包括内窥镜夹点活检、十二指肠抽吸物、支气管肺泡灌洗、腹股沟淋巴结活检、阴道活检和阴道冲洗)。第五次免疫于2009年11月16日通过肌肉注射进行，随后像以前一样立即进行电穿孔接种。分别在免疫前、免疫后1周和免疫后3周采集血样和粪便样本。免疫后3周采集组织标本。 实验2： 2009年9月，向杜兰资源分配委员会提交了一份申请，要求获得24只雌性印第安猕猴，用于该项目的下一次实验。10月分配了6只动物，12月分配了5只动物。我们目前正在等待剩下的13只动物开始实验工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Experiment 1: Twenty female Indian origin Rhesus macaques were assigned in 2008 to one of four experimental groups. All animals were previously immunized four times with DNA and various chemokines. Based on immunological data obtained from samples collected during the first four immunizations, it was decided to administer a fifth immunization. This immunization would be modified to include SIV nef/rev and H1N1 HA antigens. Three weeks prior to the fifth immunization (October 26-30, 2009), blood, fecal and BAL samples were collected from all 20 animals and shipped to UPenn. Tissue samples (to include endoscopic pinch biopsies, duodenal aspirates, bronchoaveolar lavages, inguinal lymph node biopsies, vaginal biopsies and vaginal washes) were also collected at this time. The fifth immunization was administered on November 16, 2009 via intramuscular injection followed immediately by electroporation as before. Blood and fecal samples were collected immediately prior to, one week post- and 3 weeks post-immunization. Tissue samples were also collected 3 weeks post-immunization. Experiment 2: A request to the Tulane Resource Allocation Committee was submitted in September 2009 for the acquisition of 24 female Indian origin Rhesus macaques for the next experiment in this project. Six animals were assigned in October and five animals were assigned in December. We are currently waiting for the remaining 13 animals to begin experimental work.