DNA VACCINE FOR INDUCTION OF MUCOSAL IMMUNITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 实验一： 2008年，20只雌性印度恒河猴被分配到四个实验组之一。 所有动物先前用DNA和各种趋化因子免疫四次。根据从前四次免疫接种期间采集的样本中获得的免疫学数据，决定进行第五次免疫接种。 这种免疫将被修改为包括SIV nef/rev和H1N1 HA抗原。 在第五次免疫前三周（2009年10月26 - 30日），从所有20只动物中采集血液、粪便和BAL样品，并运送至宾夕法尼亚大学。 此时还采集了组织样本（包括内窥镜夹捏活检、十二指肠抽吸物、支气管肺泡灌洗、腹股沟淋巴结活检、阴道活检和阴道冲洗液）。第五次免疫接种于2009年11月16日通过肌内注射进行，随后立即进行电穿孔，如前所述。 在免疫前即刻、免疫后1周和免疫后3周收集血液和粪便样品。 还在免疫后3周收集组织样品。 实验二： 2009年9月，向杜兰资源分配委员会提交了一份申请，要求获得24只雌性印度恒河猴，用于该项目的下一个实验。 10月分配了6只动物，12月分配了5只动物。 我们目前正在等待剩余的13只动物开始实验工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Experiment 1: Twenty female Indian origin Rhesus macaques were assigned in 2008 to one of four experimental groups. All animals were previously immunized four times with DNA and various chemokines. Based on immunological data obtained from samples collected during the first four immunizations, it was decided to administer a fifth immunization. This immunization would be modified to include SIV nef/rev and H1N1 HA antigens. Three weeks prior to the fifth immunization (October 26-30, 2009), blood, fecal and BAL samples were collected from all 20 animals and shipped to UPenn. Tissue samples (to include endoscopic pinch biopsies, duodenal aspirates, bronchoaveolar lavages, inguinal lymph node biopsies, vaginal biopsies and vaginal washes) were also collected at this time. The fifth immunization was administered on November 16, 2009 via intramuscular injection followed immediately by electroporation as before. Blood and fecal samples were collected immediately prior to, one week post- and 3 weeks post-immunization. Tissue samples were also collected 3 weeks post-immunization. Experiment 2: A request to the Tulane Resource Allocation Committee was submitted in September 2009 for the acquisition of 24 female Indian origin Rhesus macaques for the next experiment in this project. Six animals were assigned in October and five animals were assigned in December. We are currently waiting for the remaining 13 animals to begin experimental work.