VIRUS CHALLENGE STOCK PRODUCTION AND STORAGE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 核心是用于生产和冷冻保存病毒库存，供NIH资助的调查人员使用。核心的设计是为了避免在准备病毒库方面的重复工作和成本，特别是SIV和SIV。用TCID50在相应的细胞系中对病毒存活量进行定量。因此，核心防止了TNPRC调查人员之间不必要的重复工作和费用。该核心维护所有病毒库存的谱系记录。 2010年，TNPRC将61份病毒储存在液氮中，44份储存在超低温冰箱中。保存了一个Excel数据库，其中包含有关被冻结库存的详细信息，其中包括：冻结日期、滴定日期、TCID50、每种病毒的瓶数和确切储存位置。 在过去的一年里，CORE提供了46瓶不同的病毒库存。在这些库存中，2010年应调查员的具体要求准备了31个瓶子。今年还准备了三种不同病毒的若干库存和/或将其添加到核心。它们包括RT-Shiv、SIV、SIV杂交和HIV-2。 在过去的一年里，TNPRC将1只兔痘病毒挑战存放物储存在液氮中。该计划代表了核心的扩展，以包括TNPRC的其他病毒。兔痘是查德·罗伊博士领导的生物防御研究项目的一部分，该项目也在TNPRC微生物学部门工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The core is for the production and cryopreservation of virus stocks to be used by NIH funded investigators. The core is designed to avoid duplication of effort and cost in the preparation of virus stocks, particularly SIV and SHIV. The viral stocks were all quantified by TCID50 in the appropriate cell line. Thus, the core prevents the unnecessary duplication of effort and expense among investigators at the TNPRC. The core maintains records on the pedigree of all virus stocks. In 2010, 61 virus stocks were stored in Liquid Nitrogen and 44 in ultra low temperature freezers at the TNPRC. An Excel database with details on the frozen stocks was maintained and includes: date frozen, titration date, TCID50, the number of vials of each virus and the exact storage location. In the past year, the core has supplied 46 vials of different viral stocks. Of those stocks, 31 vials were prepared in 2010 as a result of specific investigator requests. Several stocks of three different viruses were also prepared and/or added to the core in this year. They include RT-SHIV, SIV.SIVhybrid and HIV-2. Over the past year, 1 Rabbitpox virus challenge stock was stored in Liquid Nitrogen at the TNPRC. This program represents expansion of the core to include other viruses at the TNPRC. Rabbitpox is part of a biodefense research program headed by Dr. Chad Roy also in the Division of Microbiology at the TNPRC.