VIRUS CHALLENGE STOCK PRODUCTION AND STORAGE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 核心是生产和冷冻保存病毒储备，供NIH资助的研究人员使用。 该核心旨在避免在制备病毒储备，特别是SIV和SIV方面的重复工作和费用。 在适当的细胞系中通过TCID 50对病毒储备液进行定量。 因此，核心防止了TNPRC调查人员之间不必要的重复努力和费用。 核心保存着所有病毒库的谱系记录。 2010年，61株病毒储存在液氮中，44株储存在TNPRC的超低温冷冻箱中。 保存了一个Excel数据库，其中包含冷冻贮备液的详细信息，包括：冷冻日期、滴定日期、TCID 50、每种病毒的小瓶数量和确切的储存位置。 在过去的一年里，核心已经提供了46瓶不同的病毒库存。 在这些库存中，2010年应调查员的具体要求制备了31瓶。 今年还准备了三种不同病毒的若干储备和/或将其添加到核心中。 它们包括RT-SIV、SIV、SIV hybrid和HIV-2。 在过去一年中，1份兔痘病毒攻毒贮备液储存在TNPRC的液氮中。 该计划代表了核心的扩展，以包括TNPRC的其他病毒。 兔痘是由乍得·罗伊博士领导的生物防御研究项目的一部分，该项目也在TNPRC的微生物学部门。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The core is for the production and cryopreservation of virus stocks to be used by NIH funded investigators. The core is designed to avoid duplication of effort and cost in the preparation of virus stocks, particularly SIV and SHIV. The viral stocks were all quantified by TCID50 in the appropriate cell line. Thus, the core prevents the unnecessary duplication of effort and expense among investigators at the TNPRC. The core maintains records on the pedigree of all virus stocks. In 2010, 61 virus stocks were stored in Liquid Nitrogen and 44 in ultra low temperature freezers at the TNPRC. An Excel database with details on the frozen stocks was maintained and includes: date frozen, titration date, TCID50, the number of vials of each virus and the exact storage location. In the past year, the core has supplied 46 vials of different viral stocks. Of those stocks, 31 vials were prepared in 2010 as a result of specific investigator requests. Several stocks of three different viruses were also prepared and/or added to the core in this year. They include RT-SHIV, SIV.SIVhybrid and HIV-2. Over the past year, 1 Rabbitpox virus challenge stock was stored in Liquid Nitrogen at the TNPRC. This program represents expansion of the core to include other viruses at the TNPRC. Rabbitpox is part of a biodefense research program headed by Dr. Chad Roy also in the Division of Microbiology at the TNPRC.