HIV ENV EPITOPE ENGINEERING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 2009年6月，6只雄性恒河猴被分配到两组中的一组(野生型gp120或突变型gp120)。在所有时间点进行完整的血细胞计数、血液化学检查和体格检查。 所有动物分别于2009年7月28日、2009年8月11日和2009年8月25日经直肠免疫。在每次免疫前和免疫后一周采集血样，监测免疫应答情况。根据所有动物对前三次免疫反应较差的情况，改变了免疫路线。所有动物分别于2009年9月29日、2009年10月13日和2009年10月27日经鼻腔免疫。在每次免疫前、第一次免疫后一周和第三次免疫后一周采集血样，监测免疫应答。最近，分别于2009年12月22日、2010年1月6日和2010年1月20日肌肉注射免疫动物，并在每次免疫前和免疫后一周采集血样，监测免疫反应。最后一次抽血定于2010年2月24日进行，之后这些动物将被释放回TNPRC。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Six male Rhesus macaques were assigned in June 2009 and allocated to one of two groups (wild type gp120 or mutant gp120). Complete blood counts, blood chemistries and physical exams were performed at all time points. All animals were immunized intrarectally on 7/28/09, 8/11/09, and 8/25/09. Blood samples were collected immediately prior to each immunization and one week post-immunization to monitor the immune response to immunization. Based on the poor immune response in all animals to the first three immunizations, the route of immunization was changed. All animals were immunized via the intranasal route on 9/29/09, 10/13/09 and 10/27/09. Blood samples were collected immediately prior to each immunization, one week after the first immunization and one week after the third immunization to monitor the immune response to immunization. Recently, the animals were immunized via intramuscular injection on 12/22/09, 1/6/10 and 1/20/10. Blood samples were collected immediately prior to each immunization and one week post-immunization to monitor the immune response. A final blood draw is scheduled for 2/24/10 after which the animals will be released back to the TNPRC.