HIV ENV EPITOPE ENGINEERING

HIV环境表位工程

• 批准号: 8173047
• 负责人: Preston A Marx
• 金额: $ 3.94万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173047
• 关键词: Animals; Back; Blood; Blood Chemical Analysis; Blood specimen; Complete Blood Count; Computer Retrieval of Information on Scientific Projects Database; Engineering; Epitopes; Funding; Grant; HIV Envelope Protein gp120; Immune response; Immunization; Institution; Intramuscular Injections; Macaca mulatta; Monitor; Research; Research Personnel; Resources; Route; Schedule; Source; Time; United States National Institutes of Health; base; male; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Six male Rhesus macaques were assigned in June 2009 and allocated to one of two groups (wild type gp120 or mutant gp120). Complete blood counts, blood chemistries and physical exams were performed at all time points. All animals were immunized intrarectally on 7/28/09, 8/11/09, and 8/25/09. Blood samples were collected immediately prior to each immunization and one week post-immunization to monitor the immune response to immunization. Based on the poor immune response in all animals to the first three immunizations, the route of immunization was changed. All animals were immunized via the intranasal route on 9/29/09, 10/13/09 and 10/27/09. Blood samples were collected immediately prior to each immunization, one week after the first immunization and one week after the third immunization to monitor the immune response to immunization. Recently, the animals were immunized via intramuscular injection on 12/22/09, 1/6/10 and 1/20/10. Blood samples were collected immediately prior to each immunization and one week post-immunization to monitor the immune response. A final blood draw is scheduled for 2/24/10 after which the animals will be released back to the TNPRC.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 2009年6月，6只雄性恒河猴被分配到两组（野生型gp 120或突变型gp 120）中的一组。 在所有时间点进行全血细胞计数、血生化和体格检查。 2009年7月28日、2009年8月11日和2009年8月25日，对所有动物进行直肠内免疫。 在每次免疫前即刻和免疫后一周收集血液样品以监测对免疫的免疫应答。 基于所有动物对前三次免疫接种的免疫应答均较差，改变了免疫接种途径。 所有动物均于2009年9月29日、2009年10月13日和2009年10月27日通过鼻内途径免疫。 在每次免疫前即刻、第一次免疫后一周和第三次免疫后一周收集血液样品，以监测对免疫的免疫应答。 最近，于2009年12月22日、2010年1月6日和2010年1月20日通过肌内注射对动物进行免疫接种。 在每次免疫前即刻和免疫后一周收集血液样品以监测免疫应答。 计划于2010年2月24日进行最终采血，之后将动物放回TNPRC。