HIV ENV EPITOPE ENGINEERING

HIV环境表位工程

• 批准号: 8173047
• 负责人: Preston A Marx
• 金额: $ 3.94万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173047
• 关键词: Animals; Back; Blood; Blood Chemical Analysis; Blood specimen; Complete Blood Count; Computer Retrieval of Information on Scientific Projects Database; Engineering; Epitopes; Funding; Grant; HIV Envelope Protein gp120; Immune response; Immunization; Institution; Intramuscular Injections; Macaca mulatta; Monitor; Research; Research Personnel; Resources; Route; Schedule; Source; Time; United States National Institutes of Health; base; male; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Six male Rhesus macaques were assigned in June 2009 and allocated to one of two groups (wild type gp120 or mutant gp120). Complete blood counts, blood chemistries and physical exams were performed at all time points. All animals were immunized intrarectally on 7/28/09, 8/11/09, and 8/25/09. Blood samples were collected immediately prior to each immunization and one week post-immunization to monitor the immune response to immunization. Based on the poor immune response in all animals to the first three immunizations, the route of immunization was changed. All animals were immunized via the intranasal route on 9/29/09, 10/13/09 and 10/27/09. Blood samples were collected immediately prior to each immunization, one week after the first immunization and one week after the third immunization to monitor the immune response to immunization. Recently, the animals were immunized via intramuscular injection on 12/22/09, 1/6/10 and 1/20/10. Blood samples were collected immediately prior to each immunization and one week post-immunization to monitor the immune response. A final blood draw is scheduled for 2/24/10 after which the animals will be released back to the TNPRC.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 2009年6月分配了六个男性恒河猕猴，并分配给两组之一（野生型GP120或突变GP120）。 在所有时间点都进行了完整的血液计数，血液化学和身体检查。 所有动物在7/28/09、8/11/09和8/25/09均已内部免疫。 在每次免疫之前和免疫后一周收集血液样本，以监测对免疫的免疫反应。 基于所有动物对前三个免疫的免疫反应不良，因此改变了免疫途径。 所有动物在9/29/09、10/13/09和10/27/09通过鼻内路线进行免疫。 在第一次免疫后一周和第三次免疫后一周，在每次免疫之前立即收集血液样本，以监测免疫反应对免疫的免疫反应。 最近，这些动物通过肌内注射在12/22/09、1/6/10和1/20/10进行免疫。 每次免疫之前立即收集血液样本，并在免疫后一周收集血液样本，以监测免疫反应。 最终抽血计划为2/24/10，然后将动物释放回TNPRC。