ISOLATION OF A NEW HIV-2 GROUP IN THE US

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的总体目标是评估一种新的F组艾滋病毒-2毒株在塞拉利昂的流行情况。据记录，美国有一例HIV-2-F病例起源于塞拉利昂地区，将进行调查。艾滋病毒-2-F疾病在人类中的流行病学和发病机制尚不清楚，尽管现有数据表明，它可能起源于在塞拉利昂煤烟芒果中发现的一种SIV毒株。由于上一次调查是在18年前进行的，目前缺乏关于艾滋病毒-2在人类中的流行率的数据，艾滋病毒-2-F可能代表一种新出现的艾滋病。为此，我们将对塞拉利昂北部人口中的艾滋病毒-2-F感染流行情况进行筛查。我们将使用已经在该地区得到验证的抗体检测。我们还将使用基于聚合酶链式反应的检测来筛选可能因抗体效价下降而遗漏的样本。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The overall objective of this project is to assess the prevalence of a novel strain of HIV-2 group F in Sierra Leone. There has been one case of HIV-2-F in the United States documented that originated in the area of Sierra Leone that will be surveyed. The epidemiology and pathogenesis of HIV-2-F disease in human populations is unknown although available data suggest that it may have originated from a strain of SIV found in sooty mangabeys in Sierra Leone. Data on the prevalence of HIV-2 in human populations are presently lacking as the last survey was made 18 years ago and HIV-2-F may represent an emerging AIDS. To this end we will screen the human population in Northern Sierra Leone for prevalence of HIV-2-F infections. We will use antibody assays that have already been validated in this region. We will also use PCR based testing to screen samples that may be missed due to waning antibody titers.