MICROSIMULATION OF ANTHRACIS-IMMUNE SYSTEM INTERACTION - ADJUVANT DEVELOPMENT

炭疽病-免疫系统相互作用的微观模拟 - 佐剂开发

• 批准号: 8172397
• 负责人: Thomas B Kepler
• 金额: $ 4.39万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172397
• 关键词: Adjuvant; Antigen Presentation; Apoptosis; Bacillus anthracis; Back; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Data Collection; Dermal; Development; Experimental Designs; Funding; Grant; Immune response; Immune system; Immunization; Institution; Interleukin-12; Lymphoid; Mediating; Modeling; Mus; Myelogenous; Process; Production; Research; Research Personnel; Resources; Source; United States National Institutes of Health; design; in vivo; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project was initiated to design and implement in vivo experiments to analyze immune responses in vivo in mice, within a systematically integrated process that cycles from experimental design, to data collection, to data analysis, to parameter estimation, to goodness of fit determination, to deduction from model, and back to experimental design. The data generated thus far suggest: 1) That distinct subsets of DCs sequentially migrate to the draining LNs after immunization with OVA+LPS. The caveat to this is that the change in DC numbers observed may instead reflect differences in differentiation, proliferation or apoptosis. Experiments to clarify these issues are in progress. 2) That IL-12 production is mediated predominantly by Lymphoid DCs and Langerhans DC, but by myeloid DCs or dermal DCs, suggesting a division of labor amongst the DC subsets. 3) That antigen-presentation is mediated by all DC subsets.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目旨在设计和实施体内实验，以分析小鼠体内的免疫反应，在一个系统集成的过程中，从实验设计，到数据收集，到数据分析，到参数估计，到拟合优度确定，到模型推导，再回到实验设计。 迄今为止产生的数据表明： 第一章 在用OVA+LPS免疫后，不同的DC亚群依次迁移到引流LN。需要注意的是，观察到的DC数量的变化可能反映了分化、增殖或凋亡的差异。正在进行实验以澄清这些问题。 （二） IL-12的产生主要由类树突状细胞和朗格汉斯树突状细胞介导，但由髓样树突状细胞或真皮树突状细胞介导，表明树突状细胞亚群之间的分工。 第三章 抗原呈递由所有DC亚群介导。