Biostatistical and Computational Biology

生物统计和计算生物学核心(BCB)的使命是为杜克大学所有与艾滋病相关的研究活动的内部协作和协调提供量化支持，从而增加CFAR调查人员所做研究的科学影响。我们将与CFAR的其他核心领导层密切合作，预测杜克大学艾滋病毒相关奖学金的需求，并创建适当的 统计方法和计算机基础设施以满足这些需求。我们预计Duke CFAR的关键需求领域包括： 1.社会和行为科学研究和临床试验的生物统计咨询 2.流式细胞术和分子病毒学实验室检测方法的发展和分析 3.统计和定量分析方面的辅导和教育 4.实验室和临床数据的数据管理和整合 CFAR调查人员可以使用Core来咨询统计或计算生物学问题，协作 在手稿或赠款上，通过参加统计或计算模块来提升他们的量化技能，或者 只需使用核心成员开发的软件工具即可。为了促进这种核心-核心交互， 我们采用单个成员是每个CFAR核心的主要联系人的模式，使我们能够 根据我们的技能和科学兴趣进行专业设置，同时也对 相关科学。 在CFAR的第一个供资周期中，BCB核心得到了广泛利用，并提供了关键的 流式细胞术、分子病毒学、社会和行为科学以及 临床核心。此外，BCB核心调查人员通过以下方式做出了独立的科学贡献 开发了多参数流式细胞术的创新软件，并开发了用于 推断HIV序列的自然历史，推断V(D)J重组组分 免疫球蛋白和T细胞受体DNA序列预测潜在T细胞与hl_A结合 表位，以及用于细胞跟踪、ELISA、Luminex和基因表达的增强的数据处理 微阵列结果。

The mission of the Biostatistics and Computational Biology Core (BCB) is to provide quantitative suppor for intramural collaboration and coordination of all AIDS-related research activities at Duke, thereby increasing the scientific impact of research done by CFAR investigators. We will work closely with the other CFAR Core leadership to anticipate the needs of HIV-related scholarship at Duke and create the appropriate statistical methods and computer infrastructure to fulfill these needs. Key areas that we anticipate will be critical needs for the Duke CFAR are: 1. Biostatistical consulting for social and behavioral sciences research and clinical trials 2. Laboratory assay development and analysis for flow cytometry and molecular virology 3. Mentoring and education in statistics and quantitative analysis 4. Data management and integration of laboratory and clinical data CFAR investigators can use the Core to consult about statistical or computational biology issues, collaborate on a manuscript or grant, upgrade their quantitative skills by taking a statistics or computational module, or simply make use of software tools developed by Core members. To facilitate such Core-Core interactions, we adopt a model where a single member is the primary contact for each CFAR Core, allowing us to specialize according to our skill sets and scientific interests while also becoming deeply familiar with the relevant science. During the first CFAR funding cycle, the BCB Core has been extensively utilized and has provided critical support to investigators from the Flow Cytometry, Molecular Virology, Social and Behavioral Sciences and Clinical Cores. In addition, BCB Core investigators have made independent scientific contributions through the development of innovative software for multi-parameter flow cytometry, and developed methods for inferring the natural histories of HIV sequences, inferring the V(D)J recombination components for immunoglobulin and T-cell receptor DNA sequences, predicting Hl_A binding by potential peptide T-cell epitopes, and enhanced data processing for cellular tracking, and ELISA, Luminex and gene expression microarray results.