MECHANICALLY GUIDED UROLOGICAL TISSUE REGENERATION IN VITRO

体外机械引导泌尿组织再生

• 批准号: 8360199
• 负责人: JIRO NAGATOMI
• 金额: $ 11.18万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360199
• 关键词: Biocompatible Materials; Bioreactors; Bladder; Bladder Tissue; Cells; Clinical; Exhibits; Funding; Grant; Growth; In Vitro; Mechanics; National Center for Research Resources; Phenotype; Polymers; Principal Investigator; Research; Research Infrastructure; Resources; Smooth Muscle Myocytes; Source; Stimulus; System; Technology; Time; Tissue Engineering; United States National Institutes of Health; cell growth; cost; reconstruction; scaffold; success; tissue regeneration; urologic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent clinical success in bladder tissue engineering demonstrated the feasibility of this technology to combine synthetic polymer scaffolds with host cells for bladder reconstruction. Yet, there remain several unresolved issues. For example, in-vitro culturing of bladder smooth muscle cells for a prolonged time period results in de-differentiation and loss of the contractile phenotype of these cells. Our group previously demonstrated that 3D cultures of bladder SMCs subjected to sustained tension exhibited significantly greater levels of contractile phenotype markers compared to the no-tension control. We hypothesize that exposure of 3D culture of bladder SMCs to appropriate mechanical stimuli leads to guided cell growth and retention of contractile phenotype. The long-term objective of the present study is to develop both biomaterials and a bioreactor system that guide SMC growth for use in the tissue engineering applications.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 最近膀胱组织工程的临床成功证明了该技术将合成聚合物支架与宿主细胞相结合进行膀胱重建的可行性。然而，仍有几个未解决的问题。例如，膀胱平滑肌细胞长时间的体外培养导致这些细胞的去分化和收缩表型的丧失。我们的小组之前证明，与无张力对照相比，经受持续张力的膀胱 SMC 的 3D 培养物表现出显着更高水平的收缩表型标记物。我们假设膀胱 SMC 的 3D 培养物暴露于适当的机械刺激会导致引导细胞生长和收缩表型的保留。本研究的长期目标是开发生物材料和生物反应器系统，引导 SMC 生长，用于组织工程应用。