MECHANICALLY GUIDED UROLOGICAL TISSUE REGENERATION IN VITRO

体外机械引导泌尿组织再生

• 批准号: 8168474
• 负责人: JIRO NAGATOMI
• 金额: $ 5.78万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168474
• 关键词: Biocompatible Materials; Bioreactors; Bladder; Bladder Tissue; Cells; Clinical; Computer Retrieval of Information on Scientific Projects Database; Exhibits; Funding; Grant; Growth; In Vitro; Institution; Mechanics; Phenotype; Polymers; Research; Research Personnel; Resources; Smooth Muscle Myocytes; Source; Stimulus; System; Technology; Time; Tissue Engineering; United States National Institutes of Health; cell growth; reconstruction; scaffold; success; tissue regeneration; urologic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent clinical success in bladder tissue engineering by Atala el al (1) demonstrated the feasibility of this technology to combine synthetic polymer scaffolds with host cells for bladder reconstruction. Yet, there remain several unresolved issues. For example, in-vitro culturing of bladder smooth muscle cells for a prolonged time period results in de-differentiation and loss of the contractile phenotype of these cells. Our group previously demonstrated that 3D cultures of bladder SMCs subjected to sustained tension exhibited significantly greater levels of contractile phenotype markers compared to the no-tension control (2). We hypothesize that exposure of 3D culture of bladder SMCs to appropriate mechanical stimuli leads to guided cell growth and retention of contractile phenotype. The long-term objective of the present study is to develop both biomaterials and a bioreactor system that guide SMC growth for use in the tissue engineering applications.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Atala等人（1）最近在膀胱组织工程中的临床成功证明了该技术将联合收割机合成聚合物支架与宿主细胞结合用于膀胱重建的可行性。然而，仍有几个未解决的问题。例如，膀胱平滑肌细胞的体外培养延长的时间段导致这些细胞的去分化和收缩表型的丧失。我们的小组先前证明，与无张力对照相比，持续张力下的膀胱SMC的3D培养物显示出显著更高水平的收缩表型标志物（2）。我们假设，暴露的三维培养的膀胱平滑肌细胞适当的机械刺激导致引导细胞生长和保持收缩表型。本研究的长期目标是开发生物材料和生物反应器系统，引导SMC生长用于组织工程应用。