Biomimetic Properties of Cementum and Its Interfaces

牙骨质及其界面的仿生特性

• 批准号: 7851450
• 负责人: Sunita P Ho
• 金额: $ 24.74万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7851450
• 关键词: Accounting; Address; Age; Animal Model; Anterior; Apical; Area; Arts; Atomic Force Microscopy; Award; Binding; Biocompatible Materials; Biological; Biomechanics; Biomedical Engineering; Biomimetics; Bone Resorption; Cadaver; Cell Adhesion; Cell Proliferation; Cell division; Cervix Uteri; Characteristics; Chemical Structure; Chemicals; Chondroitin ABC Lyase; Chondroitin Sulfate A; Chondroitin Sulfate C; Collagen; Collagen Fibril; Collagen Type I; Complex; Connective Tissue; Cytoskeleton; Data; Data Collection; Dehydration; Dental; Dental Cementum; Dental Enamel; Dentin; Dermatan Sulfate; Disease; Disease Progression; Engineering; Environment; Enzymes; Faculty; Female; Gender; Glycosaminoglycans; Goals; Guidelines; Hardness; Human; Hydration status; Image; Incisor; Inflammation; Interferometry; Investigation; Journals; Keratan Sulfate; Knowledge; Location; Manuscripts; Maps; Matrix Metalloproteinases; Measures; Mechanics; Memory; Mentors; Minerals; Modeling; Molar tooth; Natural regeneration; Pathway interactions; Periodontal Diseases; Periodontal Ligament; Periodontitis; Periodontium; Phase; Plant Roots; Procedures; Property; Proteoglycan; Publishing; Rattus; Research; Research Personnel; Shapes; Shock; Specimen; Structure; Techniques; Testing; Time; Tissue Engineering; Tissues; Tooth Loss; Tooth structure; United States National Institutes of Health; Variant; Width; Work; age group; alveolar bone; animal tissue; base; bone; cell behavior; cell growth; chondroitinase B; crosslink; hydrophilicity; keratan-sulfate endo-1,4-b-galactosidase; male; member; nanoscale; next generation; novel; oral tissue; programs; pyridinoline; response; scaffold; tissue regeneration

The overall purpose of the NIH Pathway to Independence Award K99/ROO application is to provide a year of support for the mentored phase that is crucial in completing the critical testing of four hypotheses, followed by manuscript submission to relevant journals. The subsequent 3 year independent phase is necessary to establish my research program and reaching my goals as a junior faculty member. It would enable me to establish myself as an independent investigator in biomaterials integration and characterization taking into account both human and comparable animal tissues, subsequently building tissues with well optimized bioengineered interfaces. This award will enable me to study the proposed specific aims defined in this proposal and expand my knowledge on building the next generation materials for tissue regeneration. The thrust of these efforts is based on a general hypothesis that the functional biomechanics of a tooth are derived from structural and chemical interactions of its different components at several hierarchical levels (macro-, micro- and nano-scales), and that these yield properties that depend on location, age, and gender. Destruction of tissues including the periodontal ligament (PDL), cementum, and bone can cause loss of teeth due to periodontitis. Key challenges include 1). Understanding degradation of the tissues associated with disease progression, and 2). Regeneration of the interfaces that bind the oral tissues together. To address the first challenge, an animal model for periodontitis will be developed and the sequential degeneration of tissues determined by studying their structure, chemical composition and mechanical properties. For the second challenge, tissue engineering (TE) can be used to create novel scaffolds. However, a major limitation of current TE procedures is limited knowledge of scaffold biomechanics. An efficient scaffold should sustain functional loads in addition to providing an environment conducive to desired cell behavior. To address these challenges, the inherent characteristics of the tissues and their interfaces must be determined. Then, they can be mimicked using TE to create appropriate scaffolds. Hence the following specific aims are defined to: 1). Investigate structure, chemical composition, mechanical properties of primary and secondary cementums, 2). Investigate structure, chemical composition, mechanical properties of cementum and its interface with root dentin, 3). Investigate strain fields using functional loads on anterior and posterior teeth as a function of age, 4). Perform a comparison studies between structure, chemical composition and mechanical properties of healthy human and rat alveolar bone, PDL, cementum, root dentin and their bimaterial interfaces. This information will be used to determine chronological changes in structure, chemical composition and mechanical properties in the rat periodontal tissues and their bimaterial interfaces during disease progression.

NIH Pathway to Independence Award K99/ROO申请的总体目的是提供 对完成四个假设的关键测试至关重要的辅导阶段提供一年的支持， 然后向相关期刊投稿。接下来的3年独立阶段是 作为一名初级教员，建立我的研究计划和实现我的目标是必要的。它将 使我能够成为生物材料整合和表征方面的独立研究者 考虑到人和可比较的动物组织，随后用孔构建组织， 优化的生物工程接口。这个奖项将使我能够研究所提出的具体目标定义 在这个建议和扩大我的知识建设下一代材料的组织再生。 这些努力的推动力是基于一个普遍的假设，即牙齿的功能生物力学是 源自其不同组分在几个层次上的结构和化学相互作用 （宏观、微观和纳米尺度），并且这些产生取决于位置、年龄和性别的特性。 包括牙周膜（PDL）、牙骨质和骨在内的组织的破坏可导致牙周组织的损失。 牙周炎引起的牙齿主要挑战包括1）。了解相关组织的降解 疾病进展;（2）。将口腔组织结合在一起的界面的再生。到 为了解决第一个挑战，将开发牙周炎的动物模型， 通过研究组织的结构、化学成分和机械性能来确定组织的退化 特性.对于第二个挑战，组织工程（TE）可以用来创建新的支架。 然而，目前TE程序的主要限制是支架生物力学知识有限。一个 有效的脚手架除了提供有利于所需的环境外， 细胞行为为了应对这些挑战，组织及其界面的固有特性 必须坚决。然后，可以使用TE来模拟它们以创建适当的支架。因此 确定了以下具体目标：1）。研究结构、化学成分、机械性能 一次和二次水泥的性能，2）。研究结构化学成分 牙骨质及其与根面牙本质界面的力学性质;研究应变场，使用 前牙和后牙上的功能负荷作为年龄的函数，4）。进行比较研究 健康人和大鼠牙槽骨的结构、化学成分和力学性能之间的关系， 牙周膜、牙骨质、牙根牙本质及其双材料界面。这些信息将用于确定 大鼠牙周组织结构、化学成分和力学性能的时间变化 组织和它们的双材料界面。

项目成果

The biomechanical characteristics of the bone-periodontal ligament-cementum complex.

• DOI: 10.1016/j.biomaterials.2010.05.024
• 发表时间: 2010-09
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Ho, Sunita P.;Kurylo, Michael P.;Fong, Tiffany K.;Lee, Stephen S. J.;Wagner, Hanoch D.;Ryder, Mark I.;Marshall, Grayson W.
• 通讯作者: Marshall, Grayson W.