RESEARCH CORE: CORE RESEARCH FACILITIES & INFRASTRUCTURE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 维护和扩展共享研究设施 BRIN/INBRE-1开发和加强了一个综合的全州研究设施系统，包括三个研究核心实验室，全州生物信息学设施和一个研究动物园，并将得到维护和加强。 将为获得这些共享资源提供便利，并提供全职专家技术援助。 INBRE的教师和学生将免除或支付用户费用。 试剂和其他材料的费用将由研究者通过小型教师发展种子赠款来支付，以便教师，博士后或学生只需提供消耗品的资金。此外，来自所有西部IDEA州的研究人员将被邀请使用这些设施 爱达荷州大学 A. 微阵列设施 1. Biopick机器人 2. BioRobotics 3000工作站 3. 微电网机器人 4. GSM 417排列器 5. GenePix 4000 a扫描仪 6. BioRad脉冲凝胶电泳 7. 高通量PCS 8. 实时PCR B。 成像核心： 1. IVIS 200（体外成像系统） 2. Confocal BioRad 1020（升级和软件） 3. 荧光激活细胞分选仪 C. 生物信息学核心： 1. 访问网格节点教室 2. 计算机实验室 3. 群集计算机 4. 机器人备份 5. 服务器 爱达荷州州立大学 D. MCRF（分子核心研究设施）： 1. MCRF ABI DNA测序仪服务合同 2. MCRF QPCR升级 3. 显微镜/血管室 4. MRCF组织处理器 博伊西州立大学 E. 蛋白质组学核心 1. Gyromax 737 R培养箱振荡器 2. Gyromax 737培养摇床 3. 质谱仪（LCQ DECA XP Plus）。采购的质谱仪设备包括以下组件： I. 质谱仪/Protemomex Wo 二. 东芝1400 XL Plus系列6 三. Proteomex工作站 4. NanoDrop Technologies ND-1000 5. Protean IEF系统 6. BioRad外部激光分子成像仪 7. 硅图形成像计算机 8. Eppendorf离心浓缩机5301 9. ALC冷藏柜 10. Nuaire Hood x 2 11. Axiovert组织培养显微镜 12. SPM 1000 HV控制电子设备，AFM 100 AFM接口模块，用于RHK控制系统，用于分子成像和DI的AIM-MI控制接口（PicoSPM & Multinode） 13. Coulter Epics XL流式细胞仪 14. Jouan冷藏柜 15. 数字仪器（Veeco Metrology group）多模式PicoForce系统 16. OLIS DM 45 k荧光分光光度计系统。包括以下组成部分： I. 止流附件  经典车型 二. Varian分光光度计 17. 卡里50酶标仪 18. 卡里50光纤微探针10 mm路径 19. Dell ™ GX 260计算机 20. ABIT 7300 PCR热循环仪 F. 仪器商店共用设施 1. LSM 510 Meta 405与Axio observer Z1共聚焦成像系统 2. BioTek微孔板清洗机 3. 柯达IS 4000 R影像站 4. Leica CM 1950 OUVVM低温恒温器

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Maintain and Expand Shared Research Facilities An integrated statewide system of Research facilities that includes three Research Core Laboratories, statewide Bioinformatics Facilities, and a Research Vivarium was developed and enhanced by BRIN/INBRE-1 and will be maintained and strengthened. Access to these shared resources will be facilitated and full-time expert technical assistance will be provided. User fees will be waived or paid for INBRE faculty and students. The cost of reagents and other materials will be met by the investigator through small faculty development seed grants so that faculty, postdocs, or students need only provide funds for consumable supplies. Additionally, researchers from all the Western IDeA states will be invited to use these facilities University of Idaho A. Microarray Facility 1. Biopick robot 2. BioRobotics 3000 workstation 3. MicroGrid Robot 4. GSM417 arrayer 5. GenePix 4000a scanner 6. BioRad pulsed-gel electophoresis 7. High throughput PCS 8. Real-time PCR B. Imaging Core: 1. IVIS 200 (In vitro Imaging System) 2. Confocal BioRad 1020 (upgrades and software) 3. FACS (Fluorescence Activated Cell Sorter) C. Bioinformatics Core: 1. Access Grid Node classrooms 2. Computer labs 3. Cluster computers 4. Robotic backup 5. Servers Idaho State University D. MCRF (Molecular Core Research Facility): 1. MCRF ABI DNA sequencer service contract 2. MCRF QPCR upgrade 3. Microscope/vessel chambers 4. MRCF Tissue processor Boise State University E. Proteomics Core 1. Gyromax 737R Incubator Shaker 2. Gyromax 737 Incubator Shaker 3. Mass Spectrometer (LCQ DECA XP Plus). Mass Spectrometer equipment purchased in the following components: i. Mass Spectrometer/Protemomex Wo ii. Toshiba 1400 XL Plus Series 6 iii. Proteomex workstation 4. NanoDrop Technologies ND-1000 5. Protean IEF System 6. BioRad External LASER Molecular Imager 7. Silicon Graphics Imaging Computer 8. Eppendorf Vacufuge Concentrator 5301 9. ALC Refrigerated Centrifuge 10. Nuaire Hood x 2 11. Axiovert Tissue Culture Microscop 12. SPM 1000HV Control Electronics, AFM 100 AFM Interface module for use with RHK Control systems, AIM-MI control interface for Molecular Imaging and DI (PicoSPM & Multinode) 13. Coulter Epics XL Flow Cytometer 14. Jouan Refrigerated Centrifuge 15. Digital Instruments (Veeco Metrology group) Multimode PicoForce system 16. OLIS DM 45k Spectroflurimetery system. The following components were included: i. Stop-flow accessory  Classic Model ii. Varian Spectrophotometer 17. Cary 50 Microplate reader 18. Cary 50 Fiber-optic microprobe 10 mm path 19. Dell OptiPlex GX260 computers 20. ABIT 7300 PCR Themocycler F. Instrumentations Shop Shared Facilities 1. LSM 510 Meta 405 with Axio observer Z1 Confocal Imaging System 2. BioTek Microplate Washer 3. Kodak IS4000R Image Station 4. Leica CM1950 OUVVM cryostat