Multi-Photon Confocal Microscope

描述（由申请人提供）：我们建议为辛辛那提儿童研究基金会（CCRF）购买一台多光子蔡司LSM 710激光共聚焦扫描显微镜，用于对活小鼠的活细胞结构和分子关联进行成像。CCRF拥有强大的发育生物学家，血液学家，神经生物学家，生物化学家和分子生物学家以及儿科临床医生社区，他们的研究计划将极大地受益于这种多光子共聚焦显微镜的可用性。本申请中包含的参与者反映了CHRF研究的研究项目和系统的多样性，包括血液学家，发育生物学家，神经学家和癌症生物学家，并包括高级和初级研究人员。这一申请也反映了研究所管理部门和教师的真正承诺，以协助新一代生物医学研究人员发展他们的研究计划。一个由七名有成就的研究人员组成的团队将成为所要求的仪器的主要用户，否则，如果没有这样的仪器，他们的NIH资助的研究将无法完成。该仪器将成为一个综合成像研究中心的一部分，该中心的核心管理人员和技术人员将在一个小型动物生物气泡中安置仪器并提供全面支持。除主要用户外，次要用户/新研究者也有机会使用该仪器进行活细胞/活动物成像研究。为此，我们获得了强有力的机构承诺，以财政支助的形式，弥补业务短缺、服务合同和消耗品供应。HEI资助机制将为仪器制造商蔡司在美国创造新的就业机会，并肯定会保留和创造辛辛那提技术支持人员的就业机会。该仪器承诺在许多功能上都是环保的，并且最适合那些NIH支持的工作将从附加值中受益匪浅的用户群体。

DESCRIPTION (provided by applicant): We propose to acquire a multi-photon ZEISS LSM710 laser confocal scanning microscope to image live cellular structures and molecular associations in live mice, for Cincinnati Children's Research Foundation (CCRF). CCRF has a strong community of developmental biologists, hematologists, neurobiologists, biochemists and molecular biologists, as well as pediatric clinicians, whose research programs will benefit enormously by the availability of this multi-photon confocal microscope. The participants included in this application reflect the diversity of the research programs and systems studied at CHRF, including hematologists, developmental biologist, neurologist, and cancer biologist, and encompass both senior and junior investigators. This application also reflects the genuine commitment on the part of the institute administration and the faculty to assist a new generation of biomedical researchers in the development of their research programs. A team of seven accomplished researchers will be the major users of the requested instrument, whose NIH funded studies would otherwise be unable to accomplish without such an instrument. This instrument will be a part of an integrated imaging research center, where instrument will be housed and fully supported by a core manager and technical staff in a small animal bio-bubble. In addition to major users, there will be opportunity for the instrument to be used by minor users/ new Investigators for live cell/live animal imaging studies. Towards this end we have obtained strong institutional commitments, in the form of financial support to cover operation shortfalls, service contract, and consumable supplies. The HEI funding mechanism will create new jobs for the instrument maker, ZEISS, in US, and will certainly retain and create employment of the technical support staff in Cincinnati. The instrument promises to be environmentally-friendly in many features and is best suited for the group of users whose NIH supported work will benefit tremendously by the added values.