Highly Multiplexed Digital Assays

描述（由申请人提供）：需要新技术来实现生物样品中生物标记物的多路分析。我们建议开发一种新的检测格式，用于同时检测和分析许多生物标志物。为了做到这一点，我们将开发试剂和方法，并使用模型系统验证该方法。如果成功，我们的长期目标是提高多路复用的水平，以实现当前方法无法实现的规模分析。

DESCRIPTION (provided by applicant): There is a need for new technologies to enable multiplexed analysis of collections of biomarkers in biological samples. We propose to develop a novel assay format for detecting and analyzing many biomarkers simultaneously. In order to do this we will develop reagents and methods and validate the approach using a model system. If successful, our long-term goal is to increase the level of multiplexing to enable analysis on a scale that is not feasible with current methods. PUBLIC HEALTH RELEVANCE: The technology we are developing is designed for detecting and analyzing large sets of biomarkers. This will be useful for detecting patterns of expression that provide information on normal and disease states. It has application in basic research and may also be useful in the diagnosis, treatment, and prevention of disease.