High-Throughput System for Protease Activity Profiling

用于蛋白酶活性分析的高通量系统

• 批准号: 8201173
• 负责人: Igor A. Kozlov
• 金额: $ 111.95万
• 依托单位: PROGNOSYS BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8201173
• 关键词: Affinity; Alzheimer' s Disease; Amino Acid Substitution; Arthritis; Biological; Biological Assay; Cardiovascular Diseases; Cell physiology; Cells; Cleaved cell; Code; Collection; Communicable Diseases; Complementary DNA; Computer software; Coupled; Custom; DNA; Data; Data Analyses; Detection; Development; Diagnosis; Disease; Drug Delivery Systems; Enzymes; Evaluation; Funding; Generations; Genetic Transcription; Government; Human; Hydrolysis; In Vitro; Individual; Link; Malignant Neoplasms; Matrix Metalloproteinases; Nerve Degeneration; Peptide Hydrolases; Peptide Library; Peptides; Performance; Persons; Phase; Procedures; Process; Production; Proteins; Proteome; Protocols documentation; RNA; Regulation; Reproducibility; Research; Robotics; Role; Screening procedure; Solutions; Structure; System; Techniques; Technology; Testing; Time; Translation Process; Translations; Validation; Variant; Work; base; cost; design; drug development; genome-wide; magnetic beads; new technology; next generation; novel; nuclease; software development

DESCRIPTION (provided by applicant): There is a need for new technologies to enable the analysis of the proteome. We propose to develop a novel technology for analyzing protease activity. Proteases are involved in the regulation of essential cellular processes and misregulation of proteolytic activity is involved in many diseases such as neurodegeneration, cardiovascular diseases, arthritis, Alzheimer's disease, cancer, and infectious diseases. PUBLIC HEALTH RELEVANCE: The protease activity profiling technology we are developing is useful for identifying new protease drug targets for drug development. It would help to elucidate the functional roles of specific proteases and may result in advances in the diagnosis and treatment of disease.

描述（由申请人提供）：需要新技术来进行蛋白质组分析。我们建议开发一种新的技术来分析蛋白酶的活性。蛋白酶参与基本细胞过程的调节，蛋白水解活性的失调参与许多疾病，如神经变性、心血管疾病、关节炎、阿尔茨海默病、癌症和传染病。 公共卫生关系：我们正在开发的蛋白酶活性分析技术可用于确定药物开发的新蛋白酶药物靶标。这将有助于阐明特定蛋白酶的功能作用，并可能导致疾病诊断和治疗的进展。