Arrays for Protease Activity Profiling

描述（由申请人提供）：需要新技术来实现蛋白酶活性的高分辨率、多重分析。这一领域的进展有可能发现细胞功能和调控的基本新见解。我们建议开发一种新的技术，敏感和特异性的分析蛋白酶活性的蛋白酶底物肽微阵列。我们将开发用于阵列制备、肽标记和检测的方法，这些方法针对高通量蛋白酶活性分析进行了优化。 公共卫生相关性：我们正在开发的蛋白酶活性分析系统将有助于破译蛋白质降解的细胞调控及其与表型的关系。它将有助于识别导致疾病的细胞调节障碍，因此可能导致疾病诊断和治疗方面的医学进步。

DESCRIPTION (provided by applicant): There is a need for new technologies to enable high-resolution, multiplexed analysis of protease activity. Advances in this area have the potential to enable the discovery of fundamental new insights into cellular function and regulation. We propose to develop a novel technology for sensitive and specific analysis of protease activity using protease substrate peptide microarrays. We will develop methods for array preparation, peptide labeling and detection that are optimized for high-throughput protease activity analysis. PUBLIC HEALTH RELEVANCE: The system we are developing for protease activity analysis will help to enable the deciphering of cellular regulation of protein degradation and the relationship to phenotype. It will help to identify disorders in cellular regulation that lead to disease, and therefore may result in medical advances in the diagnosis and treatment of disease.