High-Throughput System for Protease Activity Profiling

用于蛋白酶活性分析的高通量系统

• 批准号: 7670856
• 负责人: Igor A. Kozlov
• 金额: $ 20.14万
• 依托单位: PROGNOSYS BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7670856
• 关键词: Affinity; Amino Acids; Biological; Biological Assay; Cells; Cleaved cell; Code; Complementary DNA; Custom; DNA; DNA Library; DNA Microarray Chip; Detection; Diagnosis; Diagnostic; Disease; Drug Delivery Systems; Enzymes; Fungal Genome; Generations; Genetic Transcription; Genome; Genomics; Hydrolysis; In Vitro; Individual; Length; Link; Medical; Methods; Modeling; Oligonucleotides; Peptide Hydrolases; Peptide Synthesis; Peptides; Phase; Protein Analysis; Proteins; Proteome; Protocols documentation; Publishing; RNA; RNA Phages; Random Peptide Libraries; Role; Screening procedure; Solid; Solutions; Spottings; Structure; Substrate Specificity; Surface; System; Techniques; Technology; Time; Translation Process; Translations; Yeasts; base; chemical synthesis; cost; design; drug development; genome-wide; interest; magnetic beads; new technology; next generation; novel; nuclease; public health relevance; research study; tool

DESCRIPTION (provided by applicant): There is a need for new technologies to enable the analysis of the protein coding content of a genome. We propose to develop a novel technology for generation and screening of protease substrates. The technology is designed to enable large scale protease activity profiling studies. PUBLIC HEALTH RELEVANCE: The protease activity profiling technology we are developing will help to enable large-scale studies of proteases. This is useful for identifying new drug targets and for drug development. It would help to guide experiments to elucidate the functional roles of specific proteases and may also prove to be a sensitive diagnostic tool, and therefore may result in medical advances in the diagnosis and treatment of disease.

描述（由申请人提供）：需要新技术来分析基因组的蛋白质编码内容。我们建议开发一种新的蛋白酶底物的生成和筛选技术。该技术旨在实现大规模蛋白酶活性分析研究。公共卫生相关性：我们正在开发的蛋白酶活性分析技术将有助于蛋白酶的大规模研究。这对于确定新的药物靶点和药物开发是有用的。这将有助于指导实验阐明特定蛋白酶的功能作用，也可能被证明是一种敏感的诊断工具，因此可能导致疾病诊断和治疗方面的医学进步。