New Technology for Functional Studies of the Kinome

激酶组功能研究新技术

• 批准号: 8253555
• 负责人: Igor A. Kozlov
• 金额: $ 82.45万
• 依托单位: PROGNOSYS BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8253555
• 关键词: Algorithms; Amino Acid Sequence; Biological Assay; Breast Cancer Cell; Cancer cell line; Cells; Code; Complementary DNA; Computer software; Coupled; Custom; DNA; Data; Data Analyses; Development; Diagnosis; Diagnostic; Disease; Drug Delivery Systems; Enzymes; Evaluation; Funding; Generations; Genetic Transcription; Genome; Government; Human; Hydrolysis; In Vitro; Link; Literature; Malignant neoplasm of lung; Medical; Peptides; Performance; Persons; Phase; Phosphorylated Peptide; Phosphorylation Site; Phosphotransferases; Process; Production; Protein Analysis; Proteins; Proteome; Protocols documentation; Publishing; RNA; Reagent; Research; Robotics; Role; Screening procedure; Structure; System; Techniques; Technology; Time; Translation Process; Translations; Work; Yeasts; base; cost; design; drug development; genome-wide; interest; magnetic beads; new technology; next generation; novel; nuclease; research study; scale up; tool

DESCRIPTION (provided by applicant): There is a need for new technologies to enable the analysis of the protein coding content of a genome. We propose to develop a novel technology for generation and screening of peptide substrates for kinase activity profiling. The technology is designed to enable large scale kinase activity profiling studies. PUBLIC HEALTH RELEVANCE: The kinase substrate screening technology we are developing will help to enable large- scale studies of kinases. This is useful for identifying new drug targets and for drug development. It would help to guide experiments to elucidate the functional roles of specific kinases and may also prove to be a sensitive diagnostic tool, and therefore may result in medical advances in the diagnosis and treatment of disease.

描述（由申请人提供）：需要新技术来分析基因组的蛋白质编码内容。我们建议开发一种新的技术，用于生成和筛选激酶活性分析的肽底物。该技术旨在实现大规模激酶活性分析研究。 公共卫生相关性：我们正在开发的激酶底物筛选技术将有助于实现激酶的大规模研究。这对于确定新的药物靶点和药物开发是有用的。它将有助于指导实验，以阐明特定激酶的功能作用，也可能被证明是一种敏感的诊断工具，因此可能导致疾病诊断和治疗方面的医学进步。