Microarray Technology for Genomic Assays

用于基因组检测的微阵列技术

• 批准号: 7482795
• 负责人: MARK S. CHEE
• 金额: $ 81.07万
• 依托单位: PROGNOSYS BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-21 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7482795
• 关键词: Address; Amino Acid Sequence; Biological; Biological Assay; Biology; Church; Cloning; Cocos; DNA; DNA Resequencing; DNA Sequence; DNA biosynthesis; DNA chemical synthesis; Excision; Facility Construction Funding Category; Fungal Genome; Genes; Genome; Genomics; Goals; Homologous Gene; Human; Human Genome; Laws; Length; Methods; Microarray Analysis; Miniaturization; Numbers; Oligonucleotide Primers; Oligonucleotides; Peptide Sequence Determination; Phase; Preclinical Drug Evaluation; Preparation; Process; Range; Research Personnel; SNP genotyping; Sampling; Standards of Weights and Measures; Synthesis Chemistry; Synthetic Genes; Techniques; Technology; Testing; Today; Yeasts; base; cost; design; gene synthesis; high throughput screening; homologous recombination; new technology; novel; synthetic biology

DESCRIPTION (provided by applicant): Access to DNA sequence information on a genomic scale has enabled the design of ever-larger sets of oligonucleotides (oligos) for a wide variety of high-throughput assays. The ability to go directly from sequence information to synthetic oligos has been a cornerstone of many key advances in genomics. However, although the cost of oligo synthesis has decreased dramatically, it is still too expensive for many applications. We propose to address this major need in genomics. This Phase II proposal aims to develop a novel, inexpensive, highly flexible and efficient approach for manufacturing oligos and to demonstrate its utility in key applications in genomics.

描述（由申请人提供）：在基因组规模上获得DNA序列信息使得能够设计用于各种高通量测定的越来越大的寡核苷酸组（oligo）。直接从序列信息到合成寡核苷酸的能力一直是基因组学许多关键进展的基石。然而，尽管寡聚物合成的成本已经显著降低，但对于许多应用来说仍然太昂贵。我们建议在基因组学中解决这一主要需求。该第二阶段提案旨在开发一种新颖、廉价、高度灵活和有效的方法来制造寡核苷酸，并证明其在基因组学关键应用中的实用性。