COBRE: UND: MASS SPECTROMETRY CORE FACILITY

COBRE：UND：质谱核心设施

• 批准号: 8168377
• 负责人: Jonathan David Geiger
• 金额: $ 20.28万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168377
• 关键词: Budgets; Centers of Research Excellence; Chemicals; Commit; Computer Retrieval of Information on Scientific Projects Database; Contract Services; Core Facility; Coupled; Data Analyses; Electrons; Ensure; Equipment; Funding; Gases; Grant; Growth; Institution; Ions; Lipid Peroxidation; Liquid substance; Maintenance; Mass Spectrum Analysis; Methods; National Center for Research Resources; Performance; Procedures; Process; Prostaglandins; Research; Research Personnel; Resolution; Resources; Services; Source; Spectrometry, Mass, Electrospray Ionization; Support Groups; Techniques; Time; Training; Travel; United States National Institutes of Health; instrument; instrumentation; ionization; mass spectrometer; microbial alkaline proteinase inhibitor; nano; neuroinflammation; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Mass Spectrometry (MS) Core facility has, for the last six years, supported numerous investigators within and outside our COBRE-supported group. The objective here is to continue providing investigators at UND with a very high level of equipment access and technical support for their ongoing research efforts. The MS Core facility was built and equipped through funding provided by our COBRE I grant and the NCRR. The facility is equipped with three chromatographic/mass spectrometric instruments including (1) a high resolution electrospray-quadrupole/time of flight MS (ESI-QTOF) QSTAR SL equipped with nano-LC, (2) a ESI-triplequadrupole MS API 3000 with high performance liquid chromatograph (LC-ESI-MS/MS), and (3) a gas chromatograph with ion trap mass spectrometer (GC-ITMS) with electron and chemical ionization sources. This instrumentation will be used primarily for characterization of small biologically active molecules. Previously developed and new LC-SEI-MS/MS methods will be employed in projects including lipid peroxidation processes with a focus on trans-4-hydroxy-2-nonenal and it metabolites and the impact of prostaglandin formation in neuroinflammation. To ensure that investigators have access to the equipment and the relevant techniques and approaches, the Director will commit 100% effort, to train new users, provide regular maintenance of the instrumentation, setup and implement standard operating procedures, and provide assistance with data analysis. Currently 4 users are trained to operate independently the equipment in the MS Core facility. Because of increased need and growth of our COBRE-supported group, we will train ten additional users. The mass spectrometers coupled with LCs require regular sophisticated and costly maintenance and repairs. Thus, full service contracts that cover repairs and travel are required and have been budgeted. To enhance the use of the MS Core facility, the services of the Director will be provided and we will provide standard supplies. However, as demands for the use of the facility exceeds the level budgeted, frequent users will be required to provide their own supplies.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在过去的六年里，质谱学(MS)核心设施为我们科布雷支持的团队内外的众多调查人员提供了支持。这里的目标是继续为联合国开发计划署的调查人员提供非常高水平的设备、准入和技术支持，以支持他们正在进行的研究工作。微软核心设施是通过我们的Cobre I赠款和NCRR提供的资金建造和配备的。该设施配备了三台色质联用仪器，包括(1)配备纳米LC的高分辨率电喷雾四极杆/飞行时间质谱仪(ESI-QTOF)QSTAR SL，(2)配备高效液相色谱仪(LC-ESI-MS/MS)的ESI-三极杆MS API 3000，以及(3)带有电子和化学电离源的离子陷阱质谱仪(GC-ITMS)。该仪器将主要用于表征具有生物活性的小分子。以前开发的和新的LC-SEI-MS/MS方法将用于包括脂质过氧化过程的项目，重点是反式-4-羟基-2-壬烯醛及其代谢物以及前列腺素形成在神经炎症中的影响。为了确保调查人员能够接触到设备以及相关的技术和方法，主任将100%努力，培训新用户，定期维护仪器，建立和实施标准操作程序，并提供数据分析方面的协助。目前，有4名用户接受培训，以独立操作微软核心设施中的设备。由于我们Cobre支持的团队的需求增加和增长，我们将再培训10名用户。与LC相结合的质谱计需要定期进行复杂且昂贵的维护和维修。因此，维修和旅行方面的全面服务合同是必需的，而且已经编入预算。为了加强微软核心设施的使用，我们将提供主任的服务，我们将提供标准用品。然而，由于使用该设施的需求超过了预算水平，将要求经常使用该设施的人自己提供用品。