COBRE: UND: MASS SPECTROMETRY CORE FACILITY

COBRE：UND：质谱核心设施

• 批准号: 8360136
• 负责人: Jonathan David Geiger
• 金额: $ 22.62万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360136
• 关键词: 4 hydroxynonenal; Budgets; Centers of Research Excellence; Chemicals; Commit; Contract Services; Core Facility; Coupled; Data Analyses; Electrons; Ensure; Equipment; Funding; Gases; Grant; Growth; Ions; Lipid Peroxidation; Liquid substance; Maintenance; Mass Spectrum Analysis; Methods; National Center for Research Resources; Neurodegenerative Disorders; Performance; Principal Investigator; Procedures; Process; Prostaglandins; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Services; Signal Transduction; Source; Spectrometry, Mass, Electrospray Ionization; Support Groups; Techniques; Time; Training; Travel; United States National Institutes of Health; cost; instrument; instrumentation; ionization; mass spectrometer; microbial alkaline proteinase inhibitor; nano; neuroinflammation; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Mass Spectrometry (MS) Core facility has, for the last six years, supported numerous investigators within and outside our COBRE-supported group. The objective here is to continue providing investigators at UND with a very high level of equipment access and technical support for their ongoing research efforts. The MS Core facility was built and equipped through funding provided by our COBRE I grant and the NCRR. The facility is equipped with three chromatographic/mass spectrometric instruments including (1) a high resolution electrospray-quadrupole/time of flight MS (ESI-QTOF) QSTAR SL equipped with nano-LC, (2) a ESI-triplequadrupole MS API 3000 with high performance liquid chromatograph (LC-ESI-MS/MS), and (3) a gas chromatograph with ion trap mass spectrometer (GC-ITMS) with electron and chemical ionization sources. This instrumentation will be used primarily for characterization of small biologically active molecules. Previously developed and new LC-SEI-MS/MS methods will be employed in projects including lipid peroxidation processes with a focus on trans-4-hydroxy-2-nonenal and its metabolites and the impact of prostaglandin formation in neuroinflammation. To ensure that investigators have access to the equipment and the relevant techniques and approaches, the Director will commit 100% effort to train new users, provide regular maintenance of the instrumentation, setup and implement standard operating procedures, and provide assistance with data analysis. Currently 4 users are trained to operate independently the equipment in the MS Core facility. Because of increased need and growth of our COBRE-supported group, we will train ten additional users. The mass spectrometers coupled with LCs require regular sophisticated and costly maintenance and repairs. Thus, full service contracts that cover repairs and travel are required and have been budgeted. To enhance the use of the MS Core facility, the services of the Director will be provided and we will provide standard supplies. However, as demands for the use of the facility exceeds the level budgeted, frequent users will be required to provide their own supplies.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在过去的六年里，质谱（MS）核心设施支持了我们COBRE-supported小组内外的许多研究人员。 这里的目标是继续为UND的研究人员提供非常高水平的设备访问和技术支持，以支持他们正在进行的研究工作。 MS核心设施是通过我们的COBRE I赠款和NCRR提供的资金建造和装备的。该设施配备了三台色谱/质谱仪，包括（1）配备nano-LC的高分辨率电喷雾-四极杆/飞行时间MS（ESI-QTOF）QSTAR SL，（2）配备高效液相色谱仪（LC-ESI-MS/MS）的ESI-三重四极杆MS API 3000，以及（3）配备电子和化学电离源的气相色谱仪-离子阱质谱仪（GC-ITMS）。 该仪器将主要用于表征小的生物活性分子。 先前开发的和新的LC-SEI-MS/MS方法将用于包括脂质过氧化过程在内的项目，重点是反式-4-羟基-2-壬烯醛及其代谢物以及前列腺素形成对神经炎症的影响。 为确保调查人员能够使用设备以及相关技术和方法，主任将作出100%的努力，培训新用户，定期维护仪器，制定和执行标准作业程序，并协助进行数据分析。 目前，4名用户接受了独立操作MS核心设施中设备的培训。 由于需求的增加和我们的COBRE-supported组的增长，我们将培训十个额外的用户。 质谱仪与LC相结合需要定期进行复杂且昂贵的维护和维修。 因此，需要包括维修和旅行的全面服务合同，并已将其编入预算。 为了加强MS核心设施的使用，将提供主任的服务，我们将提供标准用品。 然而，由于使用该设施的需求超过预算水平，经常使用者将需要自己提供用品。