NOVEL METHODOLOGY FOR STUDYING OXIDATIVE METABOLISM

研究氧化代谢的新方法

• 批准号: 8169068
• 负责人: VICTOR KASSEY
• 金额: $ 0.87万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169068
• 关键词: Blood; Blood flow; Brain; Breathing; Cell Respiration; Cerebrum; Clinical; Computer Retrieval of Information on Scientific Projects Database; Custom; Detection; Diffuse; Disease; Family suidae; Funding; Gases; Grant; Hypercapnia; Institution; Literature; Magnetic Resonance Imaging; Maps; Measurement; Measures; Metabolic; Methodology; Methods; Modeling; Monitor; Oxygen; Oxygen Consumption; Physiologic pulse; Protons; Research; Research Personnel; Resolution; Resources; Signal Transduction; Source; Spectrum Analysis; Techniques; United States National Institutes of Health; Variant; Water; glucose metabolism; in vivo; meetings; novel; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The metabolic demands of the brain are met almost exclusively through metabolism of glucose. Under normal conditions, the brain consistently extracts about 50% of the oxygen in the blood. The changes in metabolic demand are matched by changes in blood flow rather than by changes in oxygen extraction. Regional variations in oxidative metabolism typically correlate well with regional variations in blood flow. The correlation is the rationale for substituting blood flow maps for oxygen consumption maps. There are no clinically used methods for the detection of oxidative metabolism in-vivo though metabolic disturbances in many diseases are important. In the past, in order to have a suitable model, 17O MRI techniques are modeled in swine to measure hemispheric cerebral metabolic rate of oxygen consumption (CMRO2) by detection of metabolically produced H217O by rapid T1¿-wieghted proton magnetic resonance imaging on a 1.5 T clinical scanner. The 17O is delivered as oxygen gas by a custom, minimal-loss, precision delivery breathing circuit and converted to H¿217O by oxidative metabolism. A high temporal resolution pulse sequence is employed to measure CMRO2. Proton measurements of signal change due to metabolically produced water are correlated with 17O in-vivo measurements. Using these techniques, the hemispheric CMRO2 in swine is estimated to be 1.23 ¿0.26 ¿mol/g/min; consistent with existing literature values. In the present study, we employed diffuse reflectance and correlation spectroscopy to monitor the response of cerebral oxygenation and blood flow to hypercapnia in swine and to compare oxygen consumption optically estimated with17O MRI measurements.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 大脑的代谢需求几乎完全通过葡萄糖的代谢来满足。 在正常情况下，大脑持续提取血液中约50%的氧气。 代谢需求的变化与血流的变化相匹配， 氧气提取氧化代谢的区域变化通常与 血流的区域变化。这种相关性是替代血流图的基本原理 氧气消耗图。目前尚无临床上使用的方法来检测 尽管代谢紊乱在许多疾病中是重要的，但体内代谢是重要的。过去在 为了获得合适的模型，17 O MRI技术在猪中建模以测量半球 脑氧代谢率（CMRO 2）通过检测代谢产生的 H217 O通过在1.5 T临床扫描仪上进行快速T1加权质子磁共振成像。的 17 O作为氧气通过定制的、最小损失的、精确输送的呼吸回路输送， 通过氧化代谢转化为H 217 O。一种高时间分辨率脉冲序列， 用于测量CMRO 2。由于代谢产生的信号变化的质子测量 水与17 O体内测量相关。利用这些技术， 猪的CMRO 2估计为1.23 <$0.26 <$mol/g/min;与现有文献一致 价值观在本研究中，我们采用漫反射和相关光谱， 监测猪脑氧合和血流量对高碳酸血症的反应， 氧耗用17 O MRI测量光学估计。