NOVEL METHODOLOGY FOR STUDYING OXIDATIVE METABOLISM

研究氧化代谢的新方法

• 批准号: 8361978
• 负责人: VICTOR KASSEY
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361978
• 关键词: Blood; Blood flow; Brain; Breathing; Cell Respiration; Cerebrum; Clinical; Custom; Data; Detection; Diffuse; Disease; Family suidae; Funding; Future; Gases; Grant; Hypercapnia; Lead; Literature; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Measurement; Measures; Metabolic; Methodology; Methods; Modeling; Monitor; National Center for Research Resources; Optics; Oxygen; Oxygen Consumption; Physiologic pulse; Principal Investigator; Protons; Research; Research Infrastructure; Resolution; Resources; Signal Transduction; Source; Spectrum Analysis; Techniques; Technology; United States National Institutes of Health; Variant; Water; Work; cost; glucose metabolism; human disease; in vivo; meetings; novel; optical imaging; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The metabolic demands of the brain are met almost exclusively through metabolism of glucose. Under normal conditions, the brain consistently extracts about 50% of the oxygen in the blood. The changes in metabolic demand are matched by changes in blood flow rather than by changes in oxygen extraction. Regional variations in oxidative metabolism typically correlate well with regional variations in blood flow. The correlation is the rationale for substituting blood flow maps for oxygen consumption maps. There are no clinically used methods for detection of oxidative metabolism in-vivo though metabolic disturbances in many diseases are important. In the past, in order to have a suitable model, 17O MRI techniques are modeled in swine to measure hemispheric cerebral metabolic rate of oxygen consumption (CMRO2) by detection of metabolically produced H217O by rapid T1¿-wieghted proton magnetic resonance imaging on a 1.5 T clinical scanner. The 17O is delivered as oxygen gas by a custom built, minimal-loss, precision delivery breathing circuit and converted to H217O by oxidative metabolism. A high temporal resolution pulse sequence is employed to measure CMRO2. Proton measurements of signal change due to metabolically produced water are correlated with 17O in-vivo measurements. Using these techniques, the hemispheric CMRO2 in swine is estimated to be 1.23 ¿0.26 ¿mol/g/min; consistent with existing literature values. In the present study, we employed diffuse reflectance and correlation spectroscopy to monitor the response of cerebral oxygenation and blood flow to hypercapnia in swine and to compare oxygen consumption optically estimated with17O MRI measurements. Currently, both optical and indirect detection of 17O by T1¿-wieghted proton magnetic resonance imaging studies are in progress to correlate the data. All the technology used to perform these CMRO2 estimates can easily be adapted to clinical MR scanners and it is expected that this work will lead to future studies on human diseases.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 大脑的代谢需求几乎完全通过葡萄糖的代谢来满足。 在正常情况下，大脑持续提取血液中约50%的氧气。 代谢需求的变化与血流的变化相匹配， 氧气提取氧化代谢的区域变化通常与 血流的区域变化。这种相关性是替代血流图的基本原理 氧气消耗图。目前尚无临床上使用的氧化性 尽管代谢紊乱在许多疾病中是重要的，但体内代谢是重要的。在过去， 为了有一个合适的模型，17 O MRI技术在猪中建模，以测量 大脑半球耗氧代谢率（CMRO 2）检测 代谢产生的H217 O通过快速T1加权质子磁共振成像在一个 1.5 T型临床扫描仪。17 O以氧气的形式通过一个定制的、损耗最小的 精密输送呼吸回路，并通过氧化代谢转化为H217 O。高 采用时间分辨率脉冲序列测量CMRO 2。质子测量 由代谢产生水引起的信号变化与体内17 O相关 测量.使用这些技术，猪的半球CMRO 2估计为 1.23<$0.26 <$mol/g/min;与现有文献值一致。本研究 采用漫反射光谱和相关光谱监测大脑的反应， 氧合和血流量对猪高碳酸血症的影响，并比较氧耗 用17 O MRI测量进行光学估计。目前，光学和间接检测 17 O的T1加权质子磁共振成像研究正在进行中， 关联数据。用于执行这些CMRO 2估计的所有技术都可以很容易地 适用于临床MR扫描仪，预计这项工作将导致未来的研究， 人类疾病。