MITOCHONDRIAL REDOX IMAGING AND T1RHO MRI OF BREAST CANCER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 低温NADH/FP(还原型烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白) 荧光成像或“氧化还原扫描”已经被开发用来成像体内的线粒体 基于氧化还原比率(FP/NADH或FP/(NADH+FP))的组织的氧化还原状态，并且可以 鉴于线粒体的核心作用，有助于临床诊断和治疗疾病 细胞代谢、细胞凋亡和信号转导。以前我们已经应用了这一点 不同转移潜能的人黑色素瘤小鼠移植瘤的制作技术 确定FP氧化还原比率与这些疾病的侵袭性高度相关 黑色素瘤。我们还表明，T1rho-MRI可以区分三种黑色素瘤小鼠 异种移植物。在这个子项目中，我们的目标是实现1)乳腺肿瘤小鼠的氧化还原成像 具有不同转移潜能的异种移植；2)3D氧化还原成像与 3DT1rho-MRI在小鼠乳腺移植瘤中的应用；3)葡萄糖同步成像 组织中摄取和线粒体氧化还原状态；4)识别成像生物标志物 可以区分侵袭性和惰性乳腺肿瘤。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" has been developed to image the in vivo mitochondrial redox states of tissues on the basis of the redox ratios (Fp/NADH or Fp/(NADH+Fp)) and may be useful for clinical diagnosis and treatment of diseases given the central role of mitochondria in cellular metabolism, apoptosis and signaling transductions. Previously we have applied this technique to a panel of human melanoma mouse xenografts with different metastatic potential and identify Fp redox ratio correlates highly significantly with the aggressiveness of these melanomas. We have also shown that T1rho-MRI may differentiate three melanoma mouse xenografts. In this subproject we aim to achieve 1) redox imaging of breast tumor mouse xenografts with different metastatic potentials; 2) correlation of 3D redox imaging with 3D T1rho-MRI of breast tumors in mouse xenografts; 3) simultaneous imaging of glucose uptake and mitochondrial redox state in tissue; 4) identification of imaging biomarkers that can differentiate between aggressive and indolent breast tumors.