REDOX IMAGING OF STEM CELLS

干细胞的氧化还原成像

• 批准号: 8169088
• 负责人: LIN LI
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169088
• 关键词: Apoptosis; Cardiovascular Diseases; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Disease; Flavoproteins; Funding; Grant; Image; Institution; Malignant Neoplasms; Mediator of activation protein; Metabolism; Mitochondria; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Parkinson Disease; Pathology; Reactive Oxygen Species; Regulation; Reporting; Research; Research Personnel; Resources; Scanning; Signal Transduction; Source; Stem Cell Research; Stem cells; United States National Institutes of Health; cold temperature; fluorescence imaging; in vivo; pluripotency

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cellular metabolism and redox state have been shown as key mediators in cellular metabolism, apoptosis and signaling transductions. Redox state information is useful for understanding pathology of diseases including cancer, diabetes, cardiovascular disease, and Parkinson's disease etc. In addition, the regulation of reactive oxygen species and redox state has been implicated in stem cell research. However, no direct observation of mitochondrial NAD-coupled redox state in stem cells has been reported. In this project we aim to employ the low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" to image the in vivo mitochondrial redox states of stem cells and to investigate if there is any connection between mitochondrial redox state and pluripotency of stem cells.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞代谢和氧化还原状态被认为是细胞代谢的关键调节因子， 细胞凋亡和信号转导。氧化还原状态信息有助于理解 癌症、糖尿病、心血管疾病和帕金森氏症等疾病的病理学 疾病等。此外，活性氧物种和氧化还原状态的调节 与干细胞研究有关。然而，没有直接观察到线粒体NAD偶联 干细胞中的氧化还原状态已有报道。在这个项目中，我们的目标是使用Low 温度NADH/FP(还原型烟酰胺腺嘌呤二核苷酸/氧化黄素蛋白) 荧光成像或氧化还原扫描成像体内线粒体的氧化还原状态 并研究线粒体氧化还原状态之间是否存在联系 干细胞的多能性。