REDOX IMAGING OF STEM CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞代谢和氧化还原状态已被证明是细胞代谢中的关键介质， 细胞凋亡和信号转导。氧化还原状态信息有助于理解 包括癌症、糖尿病、心血管疾病和帕金森病在内的疾病的病理学 疾病等。此外，活性氧自由基和氧化还原状态的调节也已被 与干细胞研究有关然而，没有直接观察到线粒体NAD偶联的 已经报道了干细胞中的氧化还原状态。 在这个项目中，我们的目标是雇用低 温度NADH/Fp（还原型烟酰胺腺嘌呤二核苷酸/氧化型黄素蛋白） 荧光成像或“氧化还原扫描”，以成像体内线粒体的氧化还原状态， 研究线粒体氧化还原状态与细胞增殖之间是否存在联系 以及干细胞的多能性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cellular metabolism and redox state have been shown as key mediators in cellular metabolism, apoptosis and signaling transductions. Redox state information is useful for understanding pathology of diseases including cancer, diabetes, cardiovascular disease, and Parkinson's disease etc. In addition, the regulation of reactive oxygen species and redox state has been implicated in stem cell research. However, no direct observation of mitochondrial NAD-coupled redox state in stem cells has been reported. In this project we aim to employ the low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" to image the in vivo mitochondrial redox states of stem cells and to investigate if there is any connection between mitochondrial redox state and pluripotency of stem cells.