REDOX IMAGING OF HUMAN LYMPHOMA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 氧化还原扫描可以基于NADH和Fp（氧化黄素蛋白）的荧光信号来测量体内组织的线粒体氧化还原状态，并且Fp氧化还原比率（Fp/（NADH+Fp））被先前的研究显示用于预测小鼠异种移植物中人黑素瘤和乳腺癌的侵袭性。氧化还原比也对细胞过程敏感，包括细胞凋亡、分化和代谢。在这个项目中，我们采用氧化还原扫描来研究人类淋巴瘤细胞培养和小鼠异种移植与化疗。我们希望更多地了解线粒体氧化还原状态在肿瘤进展和治疗反应中的作用。我们也为靶向线粒体氧化还原状态作为肿瘤治疗的新途径积累了证据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Redox scanning can measure the in vivo mitochondrial redox states of tissues on the basis of the fluorescence signals of NADH and Fp (oxidized flavoproteins), and Fp redox ratios (Fp/(NADH+Fp)) was shown by previous studies to predict the aggressiveness of human melanoma and breast cancer in mouse xenografts. Redox ratio is also sensitive to cellular processes including apoptosis, differentiation and metabolism. In this project we employ redox scanning to study human lymphoma cell culture and mouse xenografts with and without chemotherapy. We hope to understand more about the role of mitochondrial redox state in tumor progression and response to treatment. We may also accumulate evidence for targeting mitochondrial redox state as a novel approach of cancer therapy.