MITOCHONDRIAL REDOX STATE AS CLINICAL IMAGING BIOMARKER FOR BREAST CANCER

线粒体氧化还原状态作为乳腺癌的临床成像生物标志物

• 批准号: 8361994
• 负责人: LIN LI
• 金额: $ 1.54万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361994
• 关键词: Apoptosis; Biological Markers; Biopsy Specimen; Cancer Patient; Clinic; Clinical; Clinical Treatment; Disease; Flavoproteins; Funding; Grant; Human; Image; Magnetic Resonance; Metabolism; Mitochondria; Mus; NADH; National Center for Research Resources; Nicotinamide adenine dinucleotide; Oxidation-Reduction; Principal Investigator; Research; Research Infrastructure; Resources; Role; Scanning; Signal Transduction; Source; Techniques; Testing; Tissues; United States National Institutes of Health; Xenograft procedure; base; clinical Diagnosis; cold temperature; cost; fluorescence imaging; in vivo; malignant breast neoplasm; melanoma; optical imaging; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Low temperature NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imaging or "redox scanning" has been developed to image the in vivo mitochondrial redox states of tissues on the basis of the redox ratios (Fp/NADH or Fp/(NADH+Fp)) and may be useful for clinical diagnosis and treatment of diseases given the central role of mitochondria in cellular metabolism, apoptosis and signaling transductions. Previously we have applied this technique to human melanoma and breast cancer mouse xenografts with different metastatic potential and identify Fp redox ratio correlates highly significantly with the aggressiveness of these tumors. To further test the translational feasibility to clinic, we conducted redox scanning of clinical biopsy samples from breast cancer patients.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 低温NADH/Fp（还原型烟酰胺腺嘌呤二核苷酸/氧化型黄素蛋白）荧光成像或“氧化还原扫描”已被开发用于基于氧化还原比（Fp/NADH或Fp/（NADH+Fp））对组织的体内线粒体氧化还原状态进行成像，并且考虑到线粒体在细胞代谢、凋亡和信号转导中的中心作用，低温NADH/Fp荧光成像或“氧化还原扫描”可用于疾病的临床诊断和治疗。以前，我们已经将这种技术应用于具有不同转移潜力的人黑色素瘤和乳腺癌小鼠异种移植物，并确定Fp氧化还原比与这些肿瘤的侵袭性高度显著相关。为了进一步测试转化为临床的可行性，我们对来自乳腺癌患者的临床活检样品进行氧化还原扫描。