ANALYTICAL TECHNIQUES FOR STRUCTURAL ANALYSIS OF GLYCOSAMINOGLYCANS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 参加者将学习糖胺聚糖（GAG）结构表征的基本技术，其中包括使用适当的酶将GAG多糖解聚为二糖成分。将通过离子交换色谱法分离二糖，并通过UV检测产物。还将用2-氰基乙酰胺衍生二糖，并通过荧光检测法检测。在单独的实验中，将通过亚硝酸水解糖胺聚糖，并分析产物。还将包括用于分析糖胺聚糖衍生的二糖和寡糖的质谱技术。基本生化技术的经验是参加的先决条件 该课程将以动手实验室工作为特色，并将包括演示和讲座。将提供实验室手册，包括选定的分析技术和参考资料。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Participants will learn basic techniques for the structural characterization of glycosaminoglycans (GAGs), which will include depolymerization of the GAG polysaccharides to its disaccharide constituents using appropriate enzymes. The disaccharides will be separated by ion exchange chromatography and the products will be detected by U.V. The disaccharides will also be derivatized with 2-cyanoacetamide and will be detected with fluorescent detection. In a separate experiment, the glycosaminoglycans will be hydrolyzed by nitrous acid and the products will be analyzed. Mass spectrometry techniques for analysis of di- and oligosaccharides derived from glycosaminoglycans will also be covered. Experience with basic biochemical techniques is a prerequisite for participation The course will feature hands-on laboratory work and will include demonstrations and lectures. A lab manual including selected analytical techniques and references will be provided.