ANALYTICAL TECHNIQUES FOR STRUCTURAL ANALYSIS OF GLYCOSAMINOGLYCANS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 与会者将学习表征糖胺多聚糖(GAG)结构的基本技术，包括使用适当的酶将GAG多糖解聚为其二糖组分。二糖用离子交换层析分离，产物用紫外可见光谱检测。二糖还用2-氰基乙酰胺衍生化，用荧光检测法检测。在另一项实验中，糖胺多聚糖将被亚硝酸水解，并对产物进行分析。本课程还将介绍从糖胺多聚糖中提取的二糖和低聚糖的质谱学分析技术。基本的生化技术经验是参加的先决条件。 该课程将以动手实验室工作为特色，并包括演示和讲座。将提供一份实验室手册，包括选定的分析技术和参考资料。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Participants will learn basic techniques for the structural characterization of glycosaminoglycans (GAGs), which will include depolymerization of the GAG polysaccharides to its disaccharide constituents using appropriate enzymes. The disaccharides will be separated by ion exchange chromatography and the products will be detected by U.V. The disaccharides will also be derivatized with 2-cyanoacetamide and will be detected with fluorescent detection. In a separate experiment, the glycosaminoglycans will be hydrolyzed by nitrous acid and the products will be analyzed. Mass spectrometry techniques for analysis of di- and oligosaccharides derived from glycosaminoglycans will also be covered. Experience with basic biochemical techniques is a prerequisite for participation The course will feature hands-on laboratory work and will include demonstrations and lectures. A lab manual including selected analytical techniques and references will be provided.