NMR CHARACTERIZATION OF ACPM

ACPM 的 NMR 表征

• 批准号: 8169007
• 负责人: BRIAN G FOX
• 金额: $ 0.06万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169007
• 关键词: Acyl Carrier Protein; Computer Retrieval of Information on Scientific Projects Database; Fatty Acids; Funding; Genus Mycobacterium; Grant; Institution; Research; Research Personnel; Resources; Solutions; Source; System; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. BL21-C41(DE3) was selected as the expression host for the acyl carrier protein in mycobacteria fatty acid synthesis II system. The expression host mainly accumulate holo- and acyl-AcpM in a ~1:1 ratio. Solution NMR is used to characterize the expressed products.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 选择BL 21-C41（DE 3）作为分枝杆菌脂肪酸合成II系统中酰基载体蛋白的表达宿主。表达宿主主要以约1：1的比例积累全-和酰基-AcpM。 溶液NMR用于表征表达产物。