The Transmembrane Protein Center

跨膜蛋白中心

• 批准号: 8152104
• 负责人: BRIAN G FOX
• 金额: $ 92.96万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-30 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8152104
• 关键词: Transmembrane; Protein; Center

The Transmembrane Protein Center (TMPC) proposes to function as collaborative project within the PSIBiology Network. The TMPC research team has been assembled to provide diverse skills and experience in all constituent tasks of structural genomics. Our collective experience suggests that application of many techniques will be needed to expand the chances for success with the broad range of membrane proteins expected from participation in the PSIBiology Network. This project is well poised to provide the expertise and leadership needed to successfully advance the goals of the PSIBiology Network, including outreach to new participants in this large-scale endeavor. The assembled team includes scientists with experience in the expression, production, and characterization of membrane proteins, proven ability to solve structures of complicated membrane proteins, and a strong track record of studying the interactions of membrane proteins with their protein partners and biological ligands. The team has strong grounding in the use of bioinformatics and bioanalytical techniques. Every participant brings unique experience with the function of membrane proteins, including assay development, preparation methods, or studies with either purified proteins and living organisms. This emphasis on merging the production of membrane proteins for structure determination with the power of functional characterizations is the central philosophy of our Center. The TMPC has a primary goal of determining a founding structure for each of the unique target classes elaborated in this proposal. Our second goal is to establish an efficient membrane protein production pipeline so that increases in throughput may be progressively obtained. The TMPC expects to improve its ability to create protein variants, to refine screening based on functional and biophysical characterizations, and to improve sorting between multiple expressions pathways to find the best way produce a protein of interest. Emphasis on small-scale purification screening and further integration of automation into our efforts will increase efficiency. Expanded options for crystallization screening, access to synchrotrons, and improvements in software used to solve structures will also contribute to the increased throughput necessary to achieve PSI:Biology goals.

跨膜蛋白中心（TMPC）建议作为PSIBiology网络内的合作项目。TMPC研究团队已经组装，以提供结构基因组学的所有组成任务的各种技能和经验。我们的集体经验表明，需要应用许多技术来扩大参与PSIBiology网络预期的广泛膜蛋白的成功机会。该项目准备提供成功推进PSIBiology网络目标所需的专业知识和领导力，包括在这一大规模奋进中与新参与者的联系。组装的团队包括具有膜蛋白表达，生产和表征经验的科学家，证明有能力解决复杂膜蛋白的结构，以及研究膜蛋白与其蛋白质伴侣和生物配体相互作用的良好记录。该团队在生物信息学和生物分析技术的使用方面有很强的基础。每个参与者都带来了膜蛋白功能的独特经验，包括检测开发，制备方法或纯化蛋白质和活生物体的研究。强调将膜蛋白的生产与功能表征的能力相结合是我们中心的核心理念。TMPC的主要目标是确定本提案中详细说明的每个独特目标类别的基础结构。我们的第二个目标是建立一个有效的膜蛋白生产管道，使吞吐量的增加可以逐步获得。TMPC希望提高其创建蛋白质变体的能力，基于功能和生物物理特征来完善筛选，并改善多个表达途径之间的分选，以找到生产感兴趣蛋白质的最佳方法。强调小规模纯化筛选和进一步将自动化纳入我们的工作将提高效率。结晶筛选的扩展选项、同步加速器的使用以及用于解析结构的软件的改进也将有助于实现PSI：Biology目标所需的吞吐量增加。