STRUCTURAL ANALYSIS OF SPINACH ACYL CARRIER PROTEIN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 实验室先前的工作表明，随着连接到ACP的脂肪酸从10个碳延伸到18个碳，ACP内第30至40个残基的运动增加，如15 N HSQC峰加宽以及15 N T2弛豫增加所示。 本研究的目的是仔细表征具有15、16和17个碳脂肪酸取代基的ACP的松弛行为，因为这些可能证明ACP的稳定折叠与残基30-40的不稳定之间的过渡。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous work in the lab has shown that as the fatty acid attached to ACP elongates from 10 carbons to 18 carbons, there is an increase in the motions of residues 30 through 40 within ACP, as shown by broadening of 15N HSQC peaks as well as increasing 15N T2 relaxation. The goal of this study is to carefully characterize the relaxation behavior of ACP with 15, 16, and 17 carbon fatty acid substituents, as these are likely to demonstrate the transition between a stable fold for ACP and the destabilization of residues 30-40.