The Transmembrane Protein Center

跨膜蛋白中心(TMPC)建议作为合作项目在 PSI：生物网络。TMPC研究团队已经组建，以提供不同的技能 以及对结构基因组学所有组成任务的经验。集合的团队包括 在膜的表达、生产和表征方面有经验的科学家 蛋白质，研究膜蛋白与其蛋白质相互作用的良好记录 合作伙伴和生物配体，以及已被证明的解决蛋白质结构的能力。这支队伍有 在使用生物信息学和生物分析技术方面有扎实的基础。参与者带来 对膜蛋白的功能有独特的经验，包括分析开发， 制备方法，或用纯化的蛋白质或活体进行研究。这一强调 关于将用于结构确定的膜蛋白的生产与 功能化是我们中心的核心理念。TMPC有一个主要目标 确定本提案中阐述的四个目标类别中的每个类别的创建结构。我们的 第二个目标是建立一条高效的膜蛋白生产管道，以便增加 吞吐量可以逐步获得。TMPC希望提高其创造 蛋白质变体，根据功能和生物物理特性改进筛选，并 改进多个表达途径之间的排序，找到生产蛋白质的最佳方法 利息。强调小规模净化筛选，并进一步将自动化整合到 我们的努力将提高效率。扩大了结晶筛选的选项，可以 同步加速器，以及用于求解结构的软件的改进将有助于 提高产量以实现PSI：生物学目标。这个项目也做好了充分的准备 提供成功推进以下新兴目标所需的专业知识和领导能力 PSI：生物网络，包括向新参与者进行外联和技术转让。

The Transmembrane Protein Center (TMPC) proposes to function as collaborative project within the PSI:Biology Network. The TMPC research team has been assembled to provide diverse skills and experience in all constituent tasks of structural genomics. The assembled team includes scientists with experience in the expression, production, and characterization of membrane proteins, a strong track record of studying the interactions of membrane proteins with their protein partners and biological ligands, and proven ability to solve protein structures. The team has strong grounding in the use of bioinformatics and bioanalytical techniques. Participants bring unique experience with the function of membrane proteins, including assay development, preparation methods, or studies with either purified proteins or living organisms. This emphasis on merging the production of membrane proteins for structure determination with the power of functional characterizations is the central philosophy of our Center. The TMPC has a primary goal of determining a founding structure in each of four target classes elaborated in this proposal. Our second goal is to establish an efficient membrane protein production pipeline so that increases in throughput may be progressively obtained. The TMPC expects to improve its ability to create protein variants, to refine screening based on functional and biophysical characterizations, and to improve sorting between multiple expressions pathways to find the best way produce a protein of interest. Emphasis on small-scale purification screening and further integration of automation into our efforts will increase efficiency. Expanded options for crystallization screening, access to synchrotrons, and improvements in the software used to solve structures will contribute to the increased throughput necessary to achieve PSI:Biology goals. This project is also well poised to contribute the expertise and leadership needed to successfully advance the emerging goals of the PSI:Biology Network, including outreach and technology transfer to new participants.