The Transmembrane Protein Center

跨膜蛋白中心

• 批准号: 7982239
• 负责人: BRIAN G FOX
• 金额: $ 104.8万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-30 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7982239
• 关键词: Transmembrane; Protein; Center

The Transmembrane Protein Center (TMPC) proposes to function as collaborative project within the PSI:Biology Network. The TMPC research team has been assembled to provide diverse skills and experience in all constituent tasks of structural genomics. The assembled team includes scientists with experience in the expression, production, and characterization of membrane proteins, a strong track record of studying the interactions of membrane proteins with their protein partners and biological ligands, and proven ability to solve protein structures. The team has strong grounding in the use of bioinformatics and bioanalytical techniques. Participants bring unique experience with the function of membrane proteins, including assay development, preparation methods, or studies with either purified proteins or living organisms. This emphasis on merging the production of membrane proteins for structure determination with the power of functional characterizations is the central philosophy of our Center. The TMPC has a primary goal of determining a founding structure in each of four target classes elaborated in this proposal. Our second goal is to establish an efficient membrane protein production pipeline so that increases in throughput may be progressively obtained. The TMPC expects to improve its ability to create protein variants, to refine screening based on functional and biophysical characterizations, and to improve sorting between multiple expressions pathways to find the best way produce a protein of interest. Emphasis on small-scale purification screening and further integration of automation into our efforts will increase efficiency. Expanded options for crystallization screening, access to synchrotrons, and improvements in the software used to solve structures will contribute to the increased throughput necessary to achieve PSI:Biology goals. This project is also well poised to contribute the expertise and leadership needed to successfully advance the emerging goals of the PSI:Biology Network, including outreach and technology transfer to new participants.

跨膜蛋白中心 (TMPC) 建议作为内部合作项目 PSI：生物网络。 TMPC研究团队已集结以提供多样化的技能 以及结构基因组学所有组成任务的经验。组建的团队包括 具有膜表达、生产和表征经验的科学家 蛋白质，研究膜蛋白与其蛋白质相互作用的良好记录 合作伙伴和生物配体，以及解决蛋白质结构的经过验证的能力。团队有 在生物信息学和生物分析技术的使用方面有坚实的基础。参加者携带 在膜蛋白功能方面的独特经验，包括检测开发， 制备方法，或用纯化蛋白质或活生物体进行的研究。这个强调 将用于结构测定的膜蛋白的生产与 功能特征是我们中心的核心理念。 TMPC 有一个主要目标 确定本提案中阐述的四个目标类别中每一个目标类别的基础结构。我们的 第二个目标是建立高效的膜蛋白生产管道，从而增加 可以逐步获得吞吐量。 TMPC 希望提高其创造能力 蛋白质变体，根据功能和生物物理特征完善筛选，并 改进多种表达途径之间的排序，以找到产生蛋白质的最佳方法 兴趣。重视小规模纯化筛选，进一步融入自动化 我们的努力将提高效率。结晶筛选的扩展选项、访问 同步加速器以及用于求解结构的软件的改进将有助于 增加实现 PSI：生物学目标所需的通量。这个项目也做好了准备 贡献成功推进新兴目标所需的专业知识和领导力 PSI：生物学网络，包括向新参与者的推广和技术转让。