FATTY ACID ANALYSIS OF GANGLIOSIDES BY GC-MS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 通过酸甲醇分解从Jurkat细胞+ETOH和Jurkat细胞+ManNDAZ神经节苷脂样品制备脂肪酸甲酯（FAME）。 在100 ℃下用14%BF 3的甲醇溶液（Thermo Scientific）进行甲醇分解1小时。以C19：0为内标物，在相同的时间以与样品相同的方式水解和酯化。 用己烷萃取FAME衍生物。 通过FAME的气相色谱-质谱联用（GC-MS）进行脂肪组成分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fatty acid methyl esters (FAME) were prepared from the Jurkat cell+ETOH and Jurkat cell+ManNDAz ganglioside samples by acid methanolysis. Methanolysis was performed with 14% BF3 in methanol (Thermo Scientific) at 100oC for 1 hr. A fatty acid mixture reference standard, GLC 416 (Nu-Chek Prep, Inc.), was hydrolyzed and esterified at the same time and in the same manner as the samples with C19:0 as internal standard. The FAME derivatives were extracted with hexane. Fatty composition analysis was performed by combined gas chromatography-mass spectrometry (GC-MS) of the FAME.