PROTEINS INVOLVED IN REDOX-RELATED AND PHOTOCHEMICAL SIGNAL TRANSDUCTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们的小组将结构技术应用于信号转导中知之甚少的机制，如光和温度传感，涉及长程电子隧穿的氧化还原反应，以及大分子组装体内的合作构象变化。因此，我们研究了介导细菌趋化性的大跨膜蛋白复合物内的信号传播，研究了生物钟如何被环境所携带，并研究了一氧化氮在细菌发病机制和耐辐射性中的作用。所有这些项目的关键是蛋白质及其组装的晶体结构测定。在趋化系统中，我们确定化学感受器及其相关激酶的结构，以及鞭毛马达的接收器模块。在生物钟领域，重点是了解光传感器蛋白的结构和机制。在一氧化氮领域，我们正在确定与其催化循环相关的不同活化状态下的新型细菌一氧化氮合酶的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our group applies structural techniques to poorly understood mechanisms in signal transduction, such as light and temperature sensing, redox reactions involving long-range electron tunneling, and cooperative conformational changes within macromolecular assemblies. We have accordingly investigated signal propagation within large transmembrane protein complexes that mediate bacterial chemotaxis, pursued how circadian clocks are entrained by the environment, and studied the role of nitric oxide in bacterial pathogenesis and radiation resistance. Key to all of these projects is the crystallographic structure determination of proteins and their assemblies. In the chemotaxis system we are determining structures of chemoreceptors and their associated kinases, as well as receiver modules of the flagellar motor. In the circadian clock area with are focused on understanding the structure and mechanisms of light sensor proteins. In the nitric oxide area, we are determining structures of novel bacterial nitric oxide synthases in different activated states relevant to their catalytic cycle.