PROTEINS INVOLVED IN REDOX-RELATED AND PHOTOCHEMICAL SIGNAL TRANSDUCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的团队将结构技术应用于信号转导中知之甚少的机制，如光和温度传感、涉及远程电子隧道的氧化还原反应以及大分子组装内的协同构象变化。因此，我们研究了介导细菌趋化的大型跨膜蛋白复合体中的信号传播，探索了生物钟如何受到环境的影响，并研究了一氧化氮在细菌发病和辐射抗性中的作用。所有这些项目的关键是确定蛋白质及其组装体的晶体结构。在趋化系统中，我们正在确定化学感受器的结构及其相关的激酶，以及鞭毛马达的接收器模块。在生物钟领域，他们专注于了解光感受器蛋白质的结构和机制。在一氧化氮领域，我们正在确定与其催化循环相关的不同激活状态下的新型细菌一氧化氮合酶的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our group applies structural techniques to poorly understood mechanisms in signal transduction, such as light and temperature sensing, redox reactions involving long-range electron tunneling, and cooperative conformational changes within macromolecular assemblies. We have accordingly investigated signal propagation within large transmembrane protein complexes that mediate bacterial chemotaxis, pursued how circadian clocks are entrained by the environment, and studied the role of nitric oxide in bacterial pathogenesis and radiation resistance. Key to all of these projects is the crystallographic structure determination of proteins and their assemblies. In the chemotaxis system we are determining structures of chemoreceptors and their associated kinases, as well as receiver modules of the flagellar motor. In the circadian clock area with are focused on understanding the structure and mechanisms of light sensor proteins. In the nitric oxide area, we are determining structures of novel bacterial nitric oxide synthases in different activated states relevant to their catalytic cycle.