ANALYSIS OF PARTIALLY RECONSTITUTED KINETOCHORE

部分重建着丝粒的分析

• 批准号: 8171234
• 负责人: Trisha N. Davis
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171234
• 关键词: Animal Model; Cell Cycle; Cell Death; Cell division; Cells; Chromosome Segregation; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Congenital Abnormality; Eukaryotic Cell; Funding; Genetic Materials; Grant; Human; Institution; Kinetochores; Microtubule-Organizing Center; Microtubules; Modeling; Motor; Plus End of the Microtubule; Process; Protein Dynamics; Proteins; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Source; Structure; United States National Institutes of Health; Yeasts; design; insight; reconstitution; research study; spindle pole body; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, birth defects and cell death. Crucial to proper partitioning of the chromosomes during cell division is the establishment, arrangement and then breakdown of a bipolar spindle. The aim of this grant is to further understand the structure, assembly and dynamics of the proteins that create and control the organization of the spindle in the model organism Saccharomyces cerevisiae. The microtubule organizing center in yeast is the spindle pole body (SPB). The SPB is a dynamic structure that undergoes remodeling in a cell-cycle specific manner. We have identified proteins involved in the remodeling process and will characterize them. We have provided a detailed architectural description of the SPB and will continue our structural analysis. The SPB nucleates and organizes microtubules. We will perform a set of experiments designed to distinguish between two current models for nucleation. Finally, we have found that kinetochores can biorient without being attached to the plus-end of microtubules. New insights into biorientation will be obtained from determining the role of kinetochores, motors and other spindle proteins in the formation of the bipolar spindle when plus-end attachment is not involved. Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material.

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